Rat Phosphotylinosital 3 kinase,PI3K ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO专为大鼠样本设计的磷酸肌醇3激酶（PI3K）定量检测试剂盒，采用双抗体夹心法原理，适用于血清、血浆、组织匀浆及细胞裂解液等多种样本类型，可精准检测15.6至1000 pg/mL浓度范围内的PI3K含量。PI3K作为细胞内关键信号转导分子，通过催化磷脂酰肌醇磷酸化参与细胞增殖、分化及凋亡调控，其异常表达与肿瘤发生、代谢紊乱及免疫失调等病理过程密切相关。本试剂盒通过高特异性抗体对捕获目标蛋白，配合优化的反应体系，确保实验重复性与准确性，最低检测限达15.6 pg/mL，满足低丰度样本检测需求。该产品可广泛应用于基础科研领域，包括但不限于肿瘤微环境研究、代谢性疾病模型构建、药物作用机制探索等实验场景，为PI3K相关信号通路分析及分子调控研究提供可靠工具。所有组分均经过严格验证，开盖即用，操作流程标准化，配套详细实验方案，助力科研人员高效获取实验数据。
缩写：

PI3K
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PI3K in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-95
1:2	Average %	103
	Range %	99-107
1:4	Average %	93
	Range %	88-94
1:8	Average %	99
	Range %	95-103

回收率：

The recovery of rat PI3K spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	96	91-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.