Rat Paraoxonase 1 (PON1)ELISA Kit

Rat Paraoxonase 1 (PON1)ELISA Kit

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中文名称：

大鼠对氧磷酶-1(PON1)酶联免疫试剂盒
货号：

CSB-E14340r
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO大鼠对氧磷酶-1（PON1）酶联免疫检测试剂盒（货号：CSB-E14340r），采用双抗体夹心法定量原理，专为检测大鼠血清、血浆及组织匀浆样本中PON1活性设计，检测灵敏度达15.6 μU/mL，线性范围覆盖15.6至1000 μU/mL，适用于多种科研场景下PON1水平的精准分析。PON1是一种由肝脏合成的酯酶，在脂质代谢调节、抗氧化防御及有机磷毒物解毒过程中发挥关键作用，其表达水平与心血管疾病、代谢综合征等病理机制密切相关。本试剂盒通过预包被高特异性抗体实现目标蛋白捕获，结合优化的检测体系，可稳定检测低丰度样本，实验流程仅需4-5小时，配套提供标准品及全套试剂，支持96孔板高通量操作，适用于毒理学研究、氧化应激模型评估及代谢相关疾病的分子机制探索。产品严格验证批次间一致性，满足实验室对数据可靠性和重复性的需求，为心血管、肝脏功能及环境毒物暴露等领域的科学研究提供高效工具。
别名：

Pon1 ELISA Kit; Pon ELISA Kit; Serum paraoxonase/arylesterase 1 ELISA Kit; PON 1 ELISA Kit; EC 3.1.1.2 ELISA Kit; EC 3.1.1.81 ELISA Kit; EC 3.1.8.1 ELISA Kit; Aromatic esterase 1 ELISA Kit; A-esterase 1 ELISA Kit; Serum aryldialkylphosphatase 1 ELISA Kit
缩写：

PON1
Uniprot No.：

P55159
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 μU/mL-1000 μU/mL
灵敏度：

3.9 μU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PON1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	91
	Range %	86-95
1:400	Average %	102
	Range %	97-107
1:800	Average %	91
	Range %	85-97
1:1600	Average %	97
	Range %	91-103

回收率：

The recovery of rat PON1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μU/ml	OD1	OD2	Average	Corrected
1000	2.733	2.799	2.766	2.616
500	2.274	2.220	2.247	2.097
250	1.718	1.708	1.713	1.563
125	1.103	1.123	1.113	0.963
62.5	0.641	0.682	0.662	0.512
31.2	0.351	0.362	0.357	0.207
15.6	0.245	0.236	0.241	0.091
0	0.150	0.149	0.150

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

The potential anti-inflammatory effect of hyaluronic acid gel alone or in combination with grapefruit seed extract on induced periodontitis in mandibular molars of Wistar rats S Mostafa, AA El-Rashidy, MT Elbehwashy,Journal of Oral Biosciences,2024
Antioxidant and Anti-Inflammatory Effects of Diallyl Disulfide on Hepatotoxicity Induced by Cyclophosphamide in Rats HF Hasan,Journal Indexing and Metrics,2020
PROTECTIVE EFFECTS OF CACTUS AND/OR PAPAYA JUICES AGAINST HEPATIC AND TESTICULAR TOXICITY INDUCED BY CHLORPYRIFOS IN ALBINO RATS Mogahed H E, et al,World Journal of Pharmaceutical Research,2019
Protective effects of betanin against paracetamol and diclofenac induced neurotoxicity and endocrine disruption in rats Motawi TK, et al,Biomarkers,2019
Diosmin attenuates radiation-induced hepatic fibrosis via boosting PPAR-γ expression and hampering miR-17-5p activated canonical Wnt/β-catenin signaling Hasan H.et al,Biochemistry and Cell Biology,2016

功能：

Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification.
基因功能参考文献：
1. Compared with the rats in the control group, the serum activity of xanthine oxidase was significantly higher and that of PON1 was significantly lower in the rats in the model nonalcoholic fatty liver disease group (P<0.01). PMID: 27959408
2. Acai extract increased serum PON1 activity and reduced hepatic steatosis and hepatic injury in rats fed a high fat diet. PMID: 27642496
3. investigated the changes in plasma PON1 activity, LDL oxidation, radical scavenging activity and lipid peroxidation in D-galactose-induced aging model and also compared the results with 24-month naturally aged rats PMID: 24214524
4. Study documented, for the first time, the presence of PON3 in rat serum and, notably, found that the upregulation of PON3, rather than PON1, appears to be associated with physical training. PMID: 19091700
5. High omega-3 PUFA and ethanol decreased liver PON1 mRNA expression. Betaine restored liver PON1 mRNA expressions in low and high omega-3 PUFA ETOH groups. PMID: 20028357
6. increased N-homocysteinylation of plasma proteins in chronic hyperleptinemia caused by impairment of the animal's ability to hydrolyze homocysteine-thiolactone by PON1 PMID: 19887391
7. increased PON1 level may have a function related to the well-known atherosclerosis resistance of rats PMID: 16052486
8. caloric restriction reduced serum Paraoxonase 1 (PON1) activity and showed gender-related differences that are indicative of a different adaptive strategy of male and female rats when faced with a period of food restriction PMID: 16214326
9. Result suggests that PON1 modulation is not an integral part of the main antioxidant mechanisms of caloric restriction. PMID: 16816326
10. Serum PON1 activity increases within the first hours of fasting, representing a prompt adaptation designed to attenuate blood lipid peroxidation that cannot be sustained when fasting is prolonged PMID: 17146679
11. Delivery of the PON-1 gene may be a possible therapeutic strategy for preventing atherosclerosis. PMID: 17460375
12. Consumption of pistachio as 20% of daily caloric intake increased serum PON1 activity by 35%. PMID: 17464102
13. Serum paraoxonase activities were increased in a dose-dependent manner in taurine-treated hypothyroid rats. PMID: 17645625
14. Reduction in PON1 activity during Nippostrongylusbrasiliensis infection may be associated with inflammatory reactions. mounted against the parasites. PMID: 18458312
15. Report gender related differences in paraoxonase 1 response to high-fat diet-induced oxidative stress. PMID: 18719679
16. suggest a hepato-protective role for PON1 against inflammation, fibrosis and liver disease mediated by MCP-1. PMID: 19144177
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亚细胞定位：

Secreted, extracellular space.
蛋白家族：

Paraoxonase family
组织特异性：

Plasma. Associated with HDL.
数据库链接：

KEGG: rno:84024

STRING: 10116.ENSRNOP00000011823

UniGene: Rn.20732

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.