Rat Pancreatic Lipase,PL ELISA Kit

产品详情

产品描述：

大鼠胰脂肪酶(PL)酶联免疫试剂盒（CSB-E13784r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的PNLIP含量。PNLIP即胰腺脂肪酶，是参与脂肪消化的关键酶。其背景在于它在人体脂肪代谢中起重要作用。研究机制主要围绕其参与甘油三酯水解过程，通过特异性作用位点将甘油三酯分解为脂肪酸和甘油，以促进脂肪吸收，对其研究有助于探索脂肪代谢相关疾病。试剂盒检测范围为0.312 U/mL-20 U/mL，可满足科研领域对实验动物模型胰腺功能评估、脂肪代谢相关疾病机制研究的需求，例如在胰腺炎模型、肥胖代谢综合征或药物干预实验中监测胰脂肪酶动态变化，为营养学、药理学及基础医学研究提供稳定可靠的分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Pnlip ELISA Kit; Pancreatic triacylglycerol lipase ELISA Kit; PL ELISA Kit; Pancreatic lipase ELISA Kit; EC 3.1.1.3 ELISA Kit
缩写：

PNLIP
Uniprot No.：

P27657
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

0.312 U/mL-20 U/mL
灵敏度：

0.078 U/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	106
	Range %	103-109
1:2	Average %	96
	Range %	91-101
1:4	Average %	92
	Range %	87-97
1:8	Average %	95
	Range %	89-99

回收率：

The recovery of rat PL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	96-106
EDTA plasma (n=4)	94	87-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Plays an important role in fat metabolism. It preferentially splits the esters of long-chain fatty acids at positions 1 and 3, producing mainly 2-monoacylglycerol and free fatty acids, and shows considerably higher activity against insoluble emulsified substrates than against soluble ones.
基因功能参考文献：
1. Pnlip encoding pancreatic lipase is a possible candidate for intramuscular fat accumulation QTL in OLETF rat. PMID: 21469501
2. Regulated expression of pancreatic triglyceride lipase after rat traumatic brain injury PMID: 19760487
3. Data show that pancreatic lipase and pancreatic lipase related protein 2 gene expression is regulated anticoordinately by the amount of dietary polyunsaturated fat starting as early as the preweanling phase of development. PMID: 15181189
4. Pnlip is possible candidate for the Nidd6/of QTL in the OLETF rat PMID: 15883013
亚细胞定位：

Secreted.
蛋白家族：

AB hydrolase superfamily, Lipase family
组织特异性：

Expressed by the pancreas. Pancreatic secretory (zymogen) granule.
数据库链接：

KEGG: rno:25702

STRING: 10116.ENSRNOP00000024065

UniGene: Rn.10130