Rat P-Selectin ELISA kit

Rat P-Selectin ELISA kit

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中文名称：

大鼠P选择素(P-Selectin/CD62P)酶联免疫试剂盒
货号：

CSB-E07399r
规格：

96T/48T
价格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO大鼠P选择素（P-Selectin/CD62P）酶联免疫检测试剂盒（货号：CSB-E07399r），采用双抗体夹心法原理实现定量检测。P选择素作为选择素家族成员，主要在血小板α颗粒和内皮细胞Weibel-Palade小体中储存，在细胞激活时迅速表达于表面，介导白细胞与活化内皮或血小板的初始黏附，参与炎症反应、血栓形成及动脉粥样硬化等病理过程的研究。该试剂盒检测灵敏度为9.38 ng/mL，线性范围覆盖9.38-600 ng/mL，适用于血清、血浆及组织匀浆样本的检测，操作稳定性和重复性良好，可精准量化样本中P选择素含量。在科研领域，该试剂盒广泛用于心血管疾病模型、炎症反应机制、血栓性疾病研究及药物干预效果评估等方向，例如通过检测动物模型血液或组织中的P选择素水平，揭示其在血管内皮损伤、血小板活化等生理病理过程中的动态变化，为相关基础研究提供可靠数据支持。
别名：

Selp; P-selectin; CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; CD antigen CD62P
缩写：

SELP
Uniprot No.：

P98106
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

9.38 ng/mL-600 ng/mL
灵敏度：

2.34 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat P-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	85-99
1:2	Average %	92
	Range %	84-105
1:4	Average %	97
	Range %	92-103
1:8	Average %	94
	Range %	82-98

回收率：

The recovery of rat P-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	95-100
EDTA plasma (n=4)	95	92-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
600	2.755	2.812	2.784	2.616
300	2.217	2.236	2.227	2.059
150	1.545	1.524	1.535	1.367
75	1.051	0.979	1.015	0.847
37.5	0.587	0.622	0.605	0.437
18.75	0.456	0.438	0.447	0.279
9.38	0.288	0.279	0.284	0.116
0	0.173	0.162	0.168

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Metformin regulates the effects of IR and IGF-1R methylation on mast cell activation and airway reactivity in diabetic rats with asthma through miR-152-3p/DNMT1 axis D Fu,Cell Biology and Toxicology,2023
Docking of THPDTPI: to explore P-selectin as a common target of anti-tumor, anti-thrombotic and anti-inflammatory agent Zhu H,Oncotarget,2023
Shape memory and hemostatic silk-laponite scaffold for alveolar bone regeneration after tooth extraction trauma H Zhou,International journal of biological macromolecules,2024
DM-induced Hypermethylation of IR and IGF1R attenuates mast cell activation and airway responsiveness in rats D Fu,Journal of Cellular and Molecular Medicine,2020
Heptapeptide-based modification leading to enhancing the action of MTCA on activated platelets, P-selectin, GPIIb/IIIa Ze Li.et al,FUTURE MED CHEM,2018
Assessment of Thrombotic Risk in Atrial Fibrillation with Ultrasound Molecular Imaging of P-Selectin Jing Y.et al,Thromb Haemost,2018
Protective role of 尾-patchoulene from Pogostemon cablin against indomethacin-induced gastric ulcer in rats: Involvement of anti-inflammation Jia-Zhen Wu.et al,Phytomedicine,2017
IQCA-TAVV: To explore the effect of P-selectin, GPIIb/IIIa, IL-2, IL-6 and IL-8 on deep venous thrombosis Wu J.et al,Oncotarget,2017
ATIQCTPC: a nanomedicine capable of targeting tumor and blocking thrombosis in vivo Xu X.et al,Int J Nanomedicine.,2017
IQCA-TASS: a nano-scaled P-selectin inhibitor capable of targeting thrombus and releasing IQCA/TARGD (S) S in vivo Jianhui Wu.et al,Journal of Materials Chemistry B,2016
Impaired NO-mediated vasodilatation in rat coronary arteries after asphyxial cardiac arrest. Troelsen TT.et al,Acta Anaesthesiol Scand.,2015
Aqueous extract of Rabdosia rubescens leaves: forming nanoparticles, targeting P-selectin, and inhibiting thrombosis Wang Y. et al,Int J Nanomedicine,2015
The application of tetrahydroisoquinoline-3-carbonyl-TARGD (F) F as an anti-thrombotic agent having dual mechanisms of action Yang G et al,Mol Biosyst,2012

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功能：

Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG.
基因功能参考文献：
1. Aortic cyclooxygenase-2 and P-selectin were significantly down-regulated in plant sterols and plant sterols + tripeptides groups. PMID: 22575043
2. Fibrinogen can interact with P-selectin in order to contribute to the development of atherosclerosis, high levels of fibrinogen and P-selectin can be regarded as risk factors for markers of atherosclerosis. PMID: 22340239
3. Renal injury after ischemia/reperfusion may relate to the strong expression of P-selectin. NO may have a protective affect by decreasing the expression of P-selectin and alleviating the adhesion, aggregation and infiltration of neutrophils. PMID: 21180132
4. Both baicalin and octreotide can exert some protective effects on multiple organs of rats with severe acute pancreatitis via decreased P-selectin expression. PMID: 19780886
5. Volatile oil of Magnolia biondii can protect the kidney in rats with diabetic nephropathy by inhibiting the expressions of P-selectin protein in serum and in renal tissue. PMID: 18471420
6. P-selectin, contribute to massive infiltration of Mo into the interstitium by functioning as specialized post-capillary venules. PMID: 12081568
7. P-selectin was highly expressed on glomerular endothelial cells after injection of nephrotoxic serum.P-selectin partially mediates glomerular infiltration of macrophage in experimental crescentic glomerulonephritis. PMID: 12100021
8. Blockade of this molecule protects rat steatotic liver grafts from ischemia/reperfusion injury PMID: 12201360
9. P-selectin increases CD45-positive leukocyte activation, inflammation and neointimal formation in a Zucker diabetic rat carotid artery balloon injury model. PMID: 12377736
10. Neointimal rat SMCs supported a 2.5-fold higher arrest of monocytes and "memory" T lymphocytes than medial SMCs, which was dependent on both P-selectin and VLA-4. PMID: 14963004
11. P-selectin increased significantly in hepatic sinusoidal endothelial cells and renal tubular epithelial cells 1 h after ischemia-reperfusion PMID: 15742404
12. expression of the P-selectin gene is induced through the activation of NF-kappaB and that P-selectin may be involved in the pathogenesis of diabetic nephropathy PMID: 15888973
13. P-selectin plays a role in ischemia-reperfusion injury in experimental myocardial infarct. PMID: 17488661
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亚细胞定位：

Cell membrane; Single-pass type I membrane protein.
蛋白家族：

Selectin/LECAM family
组织特异性：

Not detected in the absence of exposure to lipopolysaccharide (LPS). Detected only after exposure to lipopolysaccharide (LPS) in the tissues examined: spleen, lung, brain, liver, heart, kidney, thymus and small intestine.
数据库链接：

KEGG: rno:25651

STRING: 10116.ENSRNOP00000003759

UniGene: Rn.10012

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.