Rat OX-2 membrane glycoprotein(CD200) ELISA kit

产品详情

产品描述：

CUSABIO大鼠OX-2膜糖蛋白（CD200）酶联免疫检测试剂盒（货号：CSB-EL004895RA），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中的CD200蛋白浓度。CD200是一种免疫调节跨膜糖蛋白，通过与其受体CD200R相互作用调控巨噬细胞和T细胞活性，在维持免疫稳态、抑制炎症反应及神经免疫调控中发挥重要作用。该试剂盒检测范围为47-3000 pg/mL，最低检测限为47 pg/mL，可精准定量多种生物样本中CD200的表达水平。其高灵敏度特性适用于科研领域中对免疫调节机制的基础研究，如炎症性疾病模型、神经退行性疾病或肿瘤微环境中的免疫逃逸机制探索，也可用于评估实验动物模型中CD200表达水平与特定病理过程的相关性。试剂盒提供标准品及预包被抗体，操作流程便捷，适用于实验室常规检测需求。
别名：

Cd200; Mox2; OX-2 membrane glycoprotein; MRC OX-2 antigen; CD antigen CD200
缩写：

CD200
Uniprot No.：

P04218
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

47 pg/mL-3000 pg/mL
灵敏度：

11.75 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CD200 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	90
	Range %	86-94
1:4	Average %	95
	Range %	90-100
1:8	Average %	92
	Range %	88-96

回收率：

The recovery of rat CD200 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	106	102-113
EDTA plasma (n=4)	98	94-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.
亚细胞定位：

Cell membrane; Single-pass type I membrane protein.
组织特异性：

Found on the surface of neurons, thymocytes, B-cells and follicular dendritic cells.
数据库链接：

STRING: 10116.ENSRNOP00000055253

UniGene: Rn.7085