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  4. Rat Neurofilament light polypeptide(NEFL) ELISA kit

Rat Neurofilament light polypeptide(NEFL) ELISA kit

文献引用 (4) 说明书下载
  • 中文名称：
    大鼠神经丝蛋白轻链多肽(NEFL)酶联免疫试剂盒
  • 货号：
    CSB-EL015688RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIOCSB-EL015688RA大鼠神经丝蛋白轻链多肽(NEFL)酶联免疫试剂盒，采用双抗体夹心法定量检测血清、血浆及组织匀浆中的NEFL含量。神经丝蛋白轻链作为神经元特异性中间纤维的核心成分，在维持轴突结构稳定性和神经信号传导中发挥重要作用，其异常表达与神经退行性疾病及创伤性神经损伤密切相关。试剂盒检测灵敏度达7.8 pg/mL，线性范围覆盖7.8-500 pg/mL，通过预包被高特异性捕获抗体与标记检测抗体的协同作用，可精准检测微量样本中的目标蛋白。适用于神经科学领域基础研究，包括中枢神经系统疾病动物模型构建、神经再生机制探索、神经毒性物质评估等场景，尤其可为脑损伤模型、脊髓病变研究及神经保护药物开发提供可靠的定量检测方案。试剂盒包含预配标准品、检测抗体及显色底物系统，支持96孔板规格，操作流程标准化且重复性良好。
  • 别名：
    Nefl ELISA Kit; Nf68 ELISA Kit; Nfl ELISA Kit; Neurofilament light polypeptide ELISA Kit; NF-L ELISA Kit; 68 kDa neurofilament protein ELISA Kit; Neurofilament triplet L protein ELISA Kit
  • 缩写：
    NEFL
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    7.8 pg/mL-500 pg/mL
  • 灵敏度：
    1.95 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat NEFL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %94
    Range %85-98
    1:2Average %107
    Range %97-111
    1:4Average %90
    Range %83-94
    1:8Average %97
    Range %91-102
  • 回收率：
    The recovery of rat NEFL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9082-94
    EDTA plasma (n=4)9991-104
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    5002.516 2.411 2.464 2.287
    2502.052 1.987 2.020 1.843
    1251.297 1.282 1.290 1.113
    62.50.725 0.784 0.755 0.578
    31.20.442 0.437 0.440 0.263
    15.60.338 0.330 0.334 0.157
    7.80.259 0.249 0.254 0.077
    00.178 0.176 0.177
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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相关问答

■ 常见问题解答
Q:

I have a customer inquiring about CSB-EL015688RA. Could you let me know whether any preservatives are contained in each component? Could you also let me know whether it was contained recombinant protein in
this kit? If yes, Please let me know expression system of the recombinant protein.

A:
Thanks for your inquiry!
CSB-EL015688RA
Standard is NS0-derived recombinant protein.
Preservative is liquid proclin 300(1:3000).
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靶点详情

  • 功能：
    Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
  • 基因功能参考文献：
    1. Furthermore, the effect of CaMKP on self-assembly of NFL was examined. Electron microscopy revealed that CaMKP markedly prevented NFL from forming large filamentous aggregates, suggesting that CaMKP-binding to NFL inhibits its filament association. These findings may provide new insights into a novel mechanism for regulating network formation of neurofilaments during neuronal differentiation. PMID: 27369073
    2. Data suggest that tetrahydropapaveroline (an endogenous catechol) causes oxidative stress resulting in astrocyte/neuronal cell death via generation of reactive oxygen species and modification/aggregation of NF-L (as in neurodegenerative diseases). PMID: 23228886
    3. In cultured cortical neurons, hamartin and tuberin co-localize with neurofilament light chain preferentially in the proximal to central growth cone region PMID: 12226091
    4. The amount of NF-L correlated with the morphological retinal and optic nerve damage in rats PMID: 15193534
    5. Increased neuritic degeneration was observed in neuronal cells overexpressing neurofilament light mutants. PMID: 15857389
    6. 68-kDa neurofilament light chain (NF-L) as identified as a binding protein of amino-terminal PH domain of PLC-gamma1. PMID: 16819285
    7. Rat pituitary cells from the proopiomelanocortin lineage nearly completely lack NF68 immunoreactivity. PMID: 16940710
    8. Data show that as a consequence of RhoA knockdown, the process which expresses neurofilament is markedly induced in C12 cells. PMID: 18556119

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  • 亚细胞定位：
    Cell projection, axon. Cytoplasm, cytoskeleton.
  • 蛋白家族：
    Intermediate filament family
  • 组织特异性：
    Expressed in the dorsal root ganglion neurons (at protein level).
  • 数据库链接：

    KEGG: rno:83613

    STRING: 10116.ENSRNOP00000018599

    UniGene: Rn.18568

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |