Rat NAD-dependent protein deacetylase sirtuin-1(SIRT1) ELISA kit

Rat NAD-dependent protein deacetylase sirtuin-1(SIRT1) ELISA kit

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中文名称：

大鼠NAD依赖性蛋白脱乙酰酶sirtuin-1(SIRT1)酶联免疫试剂盒
货号：

CSB-EL021339RA
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO专为大鼠样本设计的SIRT1酶联免疫检测试剂盒（货号：CSB-EL021339RA），采用双抗体夹心法原理实现定量检测，可精确测定血清、血浆、组织匀浆及细胞裂解液中的SIRT1蛋白水平。SIRT1作为NAD+依赖的去乙酰化酶家族核心成员，在能量代谢调控、氧化应激响应及细胞衰老等生物学过程中发挥重要作用，是代谢性疾病和衰老机制研究的重点靶标。本试剂盒检测灵敏度可达34.3 pg/mL，线性范围覆盖34.3-2200 pg/mL，可满足绝大多数样本的检测需求。预包被高亲和力捕获抗体与生物素标记检测抗体的组合确保检测特异性，实验全程包含标准品与样本同步检测的质控流程。该试剂盒适用于体外研究，如通过检测不同处理条件下（如药物干预、基因修饰）的SIRT1表达变化，探索其在能量稳态调节、神经保护或抗氧化通路中的作用机制，也可用于肥胖、糖尿病等代谢模型动物的生物标志物分析。产品提供优化后的实验缓冲体系，支持4℃短期或-20℃长期保存，开盖即用型设计显著提升实验效率。
别名：

RP11-57G10.3, SIR2L1, SIR2alpha|sir2-like 1|sirtuin 1|sirtuin type 1
缩写：

SIRT1
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

34.3 pg/mL-2200 pg/mL
灵敏度：

8.5 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Epigenetics and Nuclear Signaling
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat SIRT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-94
1:2	Average %	102
	Range %	97-105
1:4	Average %	91
	Range %	87-95
1:8	Average %	98
	Range %	92-102

回收率：

The recovery of rat SIRT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	99	95-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2200	2.357	2.272	2.315	2.209
1100	1.976	1.873	1.925	1.819
550	1.582	1.486	1.534	1.428
275	1.141	1.158	1.150	1.044
137.5	0.761	0.757	0.759	0.653
68.75	0.436	0.425	0.431	0.325
34.3	0.262	0.266	0.264	0.158
0	0.107	0.105	0.106

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Various endurance training intensities improve GFR and Up-regulate AQP2/GSK3β in lithium-induced nephropathic rats S Saberi, MA Rajizadeh, M Khaksari, A Saber,BMC nephrology,2025
Cyproheptadine causes apoptosis and decreases inflammation by disrupting thiol/disulfide balance and enhancing the levels of SIRT1 in C6 glioblastoma cells Sedat Kacar,Toxicology In Vitro,2021
Specific components of child gestures at 14 months are associated with preschoolers' language skills Y Ibara,J-STAGE,2020
Potential renoprotective effects of piceatannol in ameliorating the early-stage nephropathy associated with obesity in obese Zucker rats. Llarena M.et al,J Physiol Biochem.,2015

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.