Rat N-terminal procollagen Ⅲ propeptide,PⅢNP ELISA Kit

Rat N-terminal procollagen Ⅲ propeptide,PⅢNP ELISA Kit

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中文名称：

大鼠Ⅲ型前胶原氨基末端肽(PⅢNP)酶联免疫试剂盒
货号：

CSB-E08096r
规格：

96T/48T
价格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

大鼠Ⅲ型前胶原氨基末端肽(PⅢNP)酶联免疫试剂盒（CSB-E08096r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PⅢNP含量。PⅢNP（Ⅲ型前胶原氨基端肽）是一种与组织纤维化相关的生物标志物。它在多种疾病如慢性心力衰竭和脑积水的发展过程中扮演重要角色。研究显示，PⅢNP水平与疾病严重程度相关，可作为评估预后的指标。其具体作用机制涉及细胞外基质重塑和纤维化过程。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于科研领域对胶原代谢相关机制的研究，适用于动物模型中的纤维化疾病机制探索、抗纤维化药物研发效果评估，以及组织工程研究中胶原代谢动态监测。通过检测不同样本类型中的PⅢNP水平，研究者可深入探究胶原沉积与降解平衡在疾病发生发展中的作用，为相关科研项目提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

N-terminal procollagen 3 propeptide,P3NP,_x000D_ N-terminal procollagen III propeptide,PIIINP
缩写：

PⅢNP
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.625 ng/mL-40 ng/mL
灵敏度：

0.156 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PⅢNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-101
1:2	Average %	98
	Range %	93-102
1:4	Average %	92
	Range %	87-95
1:8	Average %	95
	Range %	91-98

回收率：

The recovery of rat PⅢNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-94
EDTA plasma (n=4)	98	94-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.102	2.077	2.090	1.959
20	1.412	1.447	1.430	1.299
10	0.924	0.945	0.935	0.804
5	0.592	0.608	0.600	0.469
2.5	0.360	0.374	0.367	0.236
1.25	0.289	0.271	0.280	0.149
0.625	0.211	0.222	0.217	0.086
0	0.130	0.132	0.131

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

MicroRNA-29a suppresses cardiac fibroblasts proliferation via targeting VEGF-A/MAPK signal pathway. Tao H.et al,Int J Biol Macromol.,2016

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.