Rat Myeloperoxidase (MPO) ELISA kit

Rat Myeloperoxidase (MPO) ELISA kit

产品详情

引用文献

产品详情

引用文献

产品评价

Most popular with customers

产品中心

信号通路

技术信息

联系信息

中文名称：

大鼠髓过氧化物酶(MPO)酶联免疫试剂盒
货号：

CSB-E08722r
规格：

96T/48T
价格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情
产品评价
引用文献

产品描述：

CUSABIO基于夹心法原理的大鼠髓过氧化物酶（Myeloperoxidase, MPO）定量检测试剂盒（货号：CSB-E08722r），适用于科研实验中血清、血浆及组织匀浆样本的MPO含量分析。MPO是中性粒细胞颗粒中特有的过氧化物酶，在先天免疫反应中催化次氯酸生成，其表达水平与炎症性疾病、心血管损伤等病理过程密切相关。CUSABIO试剂盒采用双抗体夹心ELISA技术，通过预包被的高特异性抗体实现目标蛋白捕获，线性检测范围为0.312-20 ng/mL，最低检测限可达0.078 ng/mL，具备高灵敏度和宽检测范围特性。样本处理时需注意血浆样本需添加EDTA抗凝剂，组织匀浆推荐按1:9比例稀释后离心取上清检测。该产品广泛应用于炎症机制研究、动脉粥样硬化模型评估、神经退行性疾病研究等科研领域，也可用于药物干预实验中生物标志物的动态监测。实验全程约需4.5小时，包含标准品、检测抗体、HRP酶结合物等核心组分，为研究者提供稳定可靠的定量检测方案。
缩写：

MPO
Uniprot No.：

D3ZGE2
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	94
	Range %	90-98
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-102

回收率：

The recovery of rat MPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	92	87-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.347	2.232	2.290	2.109
10	1.416	1.386	1.401	1.220
5	0.902	0.887	0.895	0.714
2.5	0.602	0.612	0.607	0.426
1.25	0.421	0.439	0.430	0.249
0.625	0.312	0.326	0.319	0.138
0.312	0.274	0.256	0.265	0.084
0	0.182	0.180	0.181

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Clinicopathological Studies on the Impact of Grape Seed Extract and L-Carnitine as Cardioprotective Agents Against Doxorubicin-Induced Toxicity in Rats TS Aldayel, OE Kilany, HNG El-Hak, HMA Abdelrazek,Life,2024
Effect of Iraqi Apricot Seed Extract on Some Physiological Parameters in Male Rats NSK Mohammad, AH Ali,Ibn AL-Haitham Journal For Pure and Applied Sciences,2024
Chitosan/hesperidin nanoparticles formulation: a promising approach against ethanol-induced gastric ulcers via Sirt1/FOXO1/PGC-1α/HO-1 pathway JA Alamoudi, TA El-Masry, MMF El-Nagar,Frontiers in Pharmacology,2024
SPH7854, a gut-limited RORγt antagonist, ameliorates TNBS-induced experimental colitis in rat Z Xiang, B Zhang, S Cao, L Cao, L Li, Q Li,International Immunopharmacology,2024
The potential antioxidant and anti-inflammatory effects of kynurenic acid on L-ornithine-induced acute pancreatitis in rats MR Allam,/,2024
DNase I targeted degradation of neutrophil extracellular traps to reduce the damage on IgAV rat XQ Chen,PloS one,2023
Donepezil Halts Acetic Acid-Induced Experimental Colitis in Rats and its Associated Cognitive Impairment Through Regulating Inflammatory/Oxidative/Apoptotic Cascades: An Add-On to its Anti-Dementia Activity EM Elbaz,SSRN,2023
Platelet-derived extracellular vesicles promote endothelial dysfunction in sepsis by enhancing neutrophil extracellular traps M Jiang,BMC immunology,2023
Neutrophil extracellular traps involved in the pathogenesis of IgA vasculitis: Confirmed in two IgAV rat models XQ Chen,PloS one,2023
Evaluating the role of polysaccharide extracted from Pleurotus columbinus on cisplatin-induced oxidative renal injury SS Mohamed,Scientific reports,2023
Nephro‐protective effect of Daphnetin in hyperoxaluria‐induced rat renal injury via alterations of the gut microbiota R Zhou,Journal of food biochemistry,2022
Protective impact of lycopene on ethinylestradiol-induced cholestasis in rats W Wadie,Naunyn-Schmiedeberg's Archives of Pharmacology,2020
C┸\phycocyanin protects against ethanol┸\induced gastric ulcers in rats: Role of HMGB1/NLRP3/NF┸\κB pathway AA Alzokaky,Basic Clin Pharmacol Toxicol,2020
Dimethyl fumarate and curcumin attenuate hepatic ischemia/reperfusion injury via Nrf2/HO-1 activation and anti-inflammatory properties Ibrahim SG,International Immunopharmacology,2020
Donor Brain Death Leads to a Worse Ischemia-Reperfusion Injury and Biliary Injury After Liver Transplantation in Rats Chen S,Transplantation Proceedings,2020
Physical training prior to myocardial infarction potentializes stem cell therapy, SDF-1/CXCR4 axis activation and inhibits the vasoconstrictor response in hypertensive rats Maximiliano I.Schaun, et al,Cytokine,2019
Microarray Analysis For Expression Profiles of lncRNAs and circRNAs in Rat Liver after Brain-Dead Donor Liver Transplantation Sanyang Chen, et al,Biomed Research International,2019
Protective effect of pentoxifylline on oxidative renal cell injury associated with renal crystal formation in a hyperoxaluric rat model Hayrettin Ozturk.et al,Urolithiasis,2018
Influence of Lycopene Administration on Neopterin, Myeloperoxidase and gamma Glutamyl Transferase in Diabetic Rats Ahmet Ufuk .et al,FRESEN ENVIRON BULL,2018
The Ameliorative Potential of Dexmedetomidine and Benincasa Cerifera Extract in Renal Ischemia/Reperfusion Injury in a Streptozotocin-Induced Diabetic Model Gehan A Hegazy.et al,Biomedical and Pharmacology,2018
Protective effect of coptisine free base on indomethacin-induced gastric ulcers in rats: Characterization of potential molecular mechanisms Luo C.et al,Life Sci,2017
Baicalin inhibits toll-like receptor 2/4 expression and downstream signaling in rat experimental periodontitis Sun JY.et al,Int Immunopharmacol.,2016
Human oral isolate Lactobacillus fermentum AGR1487 induces a pro-inflammatory response in germ-free rat colons. Anderson RC. et al,Sci Rep.,2016
The protective effects of taurine on experimental acute pancreatitis in a rat model Akay C,Hum Exp Toxicol,2013

显示更多

收起更多

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.