Rat Motilin,MTL ELISA Kit

Rat Motilin,MTL ELISA Kit

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中文名称：

大鼠胃动素(MTL)酶联免疫试剂盒
货号：

CSB-E08208r
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO专为大鼠样本设计的胃动素（MTL）酶联免疫检测试剂盒（货号：CSB-E08208r），采用双抗体夹心法定量检测血清、血浆及组织匀浆中的胃动素水平。胃动素是一种由胃肠道内分泌细胞分泌的多肽激素，通过刺激胃肠平滑肌收缩参与消化周期调控，其水平变化常被用于科研领域探索胃肠动力相关机制及疾病模型。试剂盒检测范围为31.25-2000 pg/mL，灵敏度达31.25 pg/mL，实验流程中通过预包被特异性捕获抗体与样本中的目标蛋白结合，再经生物素标记检测抗体及链霉亲和素-HRP系统显色，实现高特异性定量分析。该试剂盒适用于胃肠动力障碍动物模型研究、药物干预后胃动素分泌评估，以及消化道组织样本中MTL表达的动态监测，为胃肠生理学、病理学机制研究及药效学评价提供可靠工具。
别名：

N/A
缩写：

MTL
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MTL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-96
1:2	Average %	97
	Range %	91-105
1:4	Average %	98
	Range %	96-106
1:8	Average %	95
	Range %	89-102

回收率：

The recovery of rat MTL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	98-108
EDTA plasma (n=4)	96	92-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.725	2.905	2.815	2.614
1000	2.222	2.102	2.162	1.961
500	1.527	1.507	1.517	1.316
250	0.920	0.930	0.925	0.724
125	0.577	0.589	0.583	0.382
62.5	0.432	0.423	0.428	0.227
31.25	0.302	0.296	0.299	0.098
0	0.197	0.204	0.201

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.