Rat Membrane primary amine oxidase(AOC3) ELISA kit

Rat Membrane primary amine oxidase(AOC3) ELISA kit

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中文名称：

大鼠伯胺氧化酶膜(AOC3)酶联免疫试剂盒
货号：

CSB-EL001855RA
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO大鼠伯胺氧化酶膜（AOC3）酶联免疫检测试剂盒（货号：CSB-EL001855RA），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中AOC3的蛋白表达水平，检测灵敏度为7.8 ng/mL，线性范围覆盖7.8-500 ng/mL。AOC3是一种位于细胞膜表面的胺氧化酶，参与调控炎症反应、细胞黏附及氧化应激过程，在心血管疾病、代谢性疾病等病理机制研究中具有重要价值。本试剂盒通过特异性捕获抗体和辣根过氧化物酶标记检测抗体的协同作用，可精准定量大鼠样本中AOC3的含量，适用于体外实验中对炎症相关疾病模型、药物干预效果或基因表达调控的科研分析，尤其适合探究AOC3在组织微环境中的动态变化及其分子调控网络。试剂盒操作流程标准化，配套提供标准品和预包被酶标板，支持科研人员高效完成多样本批量检测，为炎症机制研究、靶向药物开发等基础研究提供可靠数据支持。
别名：

Aoc3; Membrane primary amine oxidase; Copper amine oxidase; Semicarbazide-sensitive amine oxidase; SSAO; VP97; Vascular adhesion protein 1; VAP-1
缩写：

AOC3
Uniprot No.：

O08590
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

7.8 ng/mL-500 ng/mL
灵敏度：

1.95 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat AOC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	84-94
1:2	Average %	96
	Range %	91-102
1:4	Average %	84
	Range %	81-88
1:8	Average %	93
	Range %	88-99

回收率：

The recovery of rat AOC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	94	91-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
500	2.075	2.086	2.081	1.931
250	1.540	1.669	1.605	1.455
125	1.111	1.156	1.134	0.984
62.5	0.758	0.750	0.754	0.604
31.2	0.437	0.465	0.451	0.301
15.6	0.261	0.277	0.269	0.119
7.8	0.211	0.223	0.217	0.067
0	0.149	0.150	0.150

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis.
基因功能参考文献：
1. Data show that 35 potential amine oxidase copper containing 3 (SSAO)-interacting proteins were found to be altered in type 2 Diabetes (T2D). PMID: 28910971
2. VAP-1 plays a critical role in the pathophysiology of renal ischemia/reperfusion injury by enhancement of neutrophil infiltration generating a local hydrogen peroxide gradient. PMID: 28318627
3. The changes of K(M) and k(c)/K(M) values of copper amine oxidase appear due to the presence of positively charged groups in the substrate molecules. PMID: 20686801
4. Provide evidence for a role for VAP-1 in the inflammatory outcome of diabetic retinopathy. Specifically, VAP-1 plays a central role in regulating leukocyte transmigration rate during chronic low-grade inflammation. PMID: 19635478
5. Our parsimonious conclusion is that there is a relation between the risk factors of AD and vascular dementia (diabetes, oxidative stress and chronic inflammation) and SSAO activity, which may originate from the vessel wall. PMID: 16524643
6. Enzymatic activity of the atypical endothelial adhesion molecule AOC3, and possibly that of other related oxidases, is crucial for leukocyte exit from the vessels in inflamed joints in vivo. PMID: 16947396
7. It was concluded that hydroxylamine exerts hypotension partly through conversion to nitric oxide and partly by a "hydralazine-like" mechanism involving SSAO inhibition PMID: 17385063
8. BZA + V treatment did not reduce HbA(1C) levels,but it reduced the elevated serum SSAO activity, decreased advanced-glycation end products and increased nitric oxide in diabetic animals. PMID: 17431735
9. evidence for an important role of VAP-1 in the recruitment of macrophages to choroidal neovascularization lesions, establishing a novel link between VAP-1 and angiogenesis PMID: 18436961
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亚细胞定位：

Membrane; Single-pass type II membrane protein.
蛋白家族：

Copper/topaquinone oxidase family
组织特异性：

Highly expressed in adipocytes, aorta and lung. Expressed at lower levels in heart, kidney, large intestine, liver, small intestine and stomach.
数据库链接：

KEGG: rno:29473

STRING: 10116.ENSRNOP00000059889

UniGene: Rn.198327

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.