Rat Matrix Metalloproteinase 14,MMP-14 ELISA Kit

产品详情

产品描述：

大鼠基质金属蛋白酶14(MMP-14)酶联免疫试剂盒（CSB-E13158r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MMP14含量。MMP14 即基质金属蛋白酶 14，在细胞外基质重塑等生理过程中发挥重要作用。其异常表达与肿瘤生长、侵袭和转移密切相关。研究聚焦于其催化机制和调控网络，旨在揭示其在疾病发生发展中的作用，为开发针对性治疗策略提供依据。试剂盒检测范围为46.88 pg/mL-3000 pg/mL，适用于探索大鼠模型中MMP-14在疾病发生发展中的作用机制，例如评估肿瘤微环境调控、组织修复异常或病理状态下的酶活性变化。可为体外实验提供定量数据支持，助力分子机制研究与药物开发。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

MMP14
Uniprot No.：

Q10739
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

46.88 pg/mL-3000 pg/mL
灵敏度：

11.75 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-14 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	88-95
1:2	Average %	95
	Range %	90-102
1:4	Average %	97
	Range %	95-107
1:8	Average %	101
	Range %	96-105

回收率：

The recovery of rat MMP-14 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-96
EDTA plasma (n=4)	98	95-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Endopeptidase that degrades various components of the extracellular matrix such as collagen. Activates progelatinase A. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15 in association with pro-MMP2. Involved in the formation of the fibrovascular tissues in association with pro-MMP2. Cleaves ADGRB1 to release vasculostatin-40 which inhibits angiogenesis.
亚细胞定位：

Membrane; Single-pass type I membrane protein. Melanosome. Cytoplasm.
蛋白家族：

Peptidase M10A family
数据库链接：

KEGG: rno:81707

STRING: 10116.ENSRNOP00000049168

UniGene: Rn.10371