Rat Macrophage Migration Inhibitory Factor,MIF ELISA Kit

Rat Macrophage Migration Inhibitory Factor,MIF ELISA Kit

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中文名称：

大鼠巨噬细胞移动抑制因子(MIF)酶联免疫试剂盒
货号：

CSB-E07293r
规格：

96T/48T
价格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO双抗体夹心法ELISA试剂盒，专用于定量检测大鼠血清、血浆及组织匀浆样本中的巨噬细胞移动抑制因子（MIF）。MIF作为重要的炎症调节因子，在免疫应答、细胞增殖及炎症性疾病中发挥关键作用，其动态变化可为感染、自身免疫疾病及肿瘤相关机制研究提供重要依据。试剂盒检测范围覆盖62.5-4000 pg/mL，灵敏度达15.6 pg/mL，采用优化的预包被抗体体系，标准品与样本仅需1.5小时孵育即可完成靶标捕获，配合TMB显色系统实现精准定量，整个检测流程可在约3小时内完成。产品包含预装冻干标准品、生物素化检测抗体及即用型显色试剂，适用于科研场景下对大鼠MIF表达水平的动态监测，例如炎症模型构建、免疫调控机制探索或药物干预效果评估等研究，实验操作兼容常规酶标仪检测，重复性强且无需复杂样本前处理，为体外实验提供高效检测方案。
别名：

Mif ELISA Kit; Macrophage migration inhibitory factor ELISA Kit; MIF ELISA Kit; EC 5.3.2.1 ELISA Kit; Glutathione-binding 13 kDa protein ELISA Kit; L-dopachrome isomerase ELISA Kit; L-dopachrome tautomerase ELISA Kit; EC 5.3.3.12 ELISA Kit; Phenylpyruvate tautomerase ELISA Kit
缩写：

MIF
Uniprot No.：

P30904
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

62.5 pg/mL-4000 pg/mL
灵敏度：

15.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	95
Range %	90-100
1:10	Average %	98
Range %	92-102
1:20	Average %	94
Range %	89-99
1:40	Average %	92
Range %	82-96

回收率：

The recovery of rat MIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-94
EDTA plasma (n=4)	96	92-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.712	2.611	2.662	2.507
2000	2.054	1.953	2.004	1.849
1000	1.286	1.307	1.297	1.142
500	0.748	0.737	0.743	0.588
250	0.478	0.467	0.473	0.318
125	0.360	0.371	0.366	0.211
62.5	0.279	0.275	0.277	0.122
0	0.155	0.154	0.155

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

The Role of Macrophage Migration Inhibitory Factor in Remote Ischemic Conditioning Induced Hepatoprotection in A Rodent Model of Liver Transplantation Emontzpohl C, et al,Canadian Journal of Cardiology,2019
Paralemmin-3 contributes to lipopolysaccharide-induced inflammatory response and is involved in lipopolysaccharide-Toll-like receptor-4 signaling in alveolar macrophages Chen XX.et al,Int J Mol Med,2017
The potential role of polymethyl methacrylate as a new packaging material for the implantable medical device in the bladder Kim SJ.et al,Biomed Res Int.,2015
Gender differences in oesophageal mucosal injury in a reflux oesophagitis model of rats Tomohiro Masaka et al,GUT,2013

功能：

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
亚细胞定位：

Secreted. Cytoplasm.
蛋白家族：

MIF family
组织特异性：

Expressed in a wide variety of organs including brain, spleen, liver, muscle and kidney.
数据库链接：

KEGG: rno:103694877

STRING: 10116.ENSRNOP00000008608

UniGene: Rn.2661

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.