Rat Lecithin Cholesterol Acyltransferase(LCAT) ELISA Kit

Rat Lecithin Cholesterol Acyltransferase(LCAT) ELISA Kit

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中文名称：

大鼠卵磷脂胆固醇酰基转移酶(LCAT)酶联免疫试剂盒
货号：

CSB-E14201r
规格：

96T/48T
价格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

大鼠卵磷脂胆固醇酰基转移酶(LCAT)酶联免疫试剂盒（CSB-E14201r）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LCAT含量。LCAT即卵磷脂胆固醇酰基转移酶，在胆固醇逆向转运中发挥关键作用。它能催化卵磷脂上的脂肪酸转移到胆固醇上形成胆固醇酯，利于胆固醇转运与代谢。相关研究围绕其在动脉粥样硬化等疾病中的作用机制，以探索潜在治疗靶点。试剂盒检测范围为1.87 ng/mL-120 ng/mL，本产品可广泛应用于基础科研领域，例如研究高脂血症、动脉粥样硬化等疾病模型中LCAT的动态变化，评估药物或基因干预对脂代谢调控的影响，以及探索胆固醇代谢相关分子机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

LcatPhosphatidylcholine-sterol acyltransferase ELISA kit; EC 2.3.1.43 ELISA kit; Lecithin-cholesterol acyltransferase ELISA kit; Phospholipid-cholesterol acyltransferase ELISA kit
缩写：

LCAT
Uniprot No.：

P18424
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.87 ng/mL-120 ng/mL
灵敏度：

0.94 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat LCAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	102
	Range %	92-107
1:200	Average %	88
	Range %	84-93
1:400	Average %	101
	Range %	94-105
1:800	Average %	102
	Range %	92-105

回收率：

The recovery of rat LCAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-96
EDTA plasma (n=4)	102	92-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
120	0.075	0.079	0.077
60	0.172	0.178	0.175
30	0.374	0.382	0.378
15	0.634	0.624	0.629
7.5	1.178	1.146	1.162
3.75	1.504	1.533	1.519
1.87	1.673	1.622	1.648
0	2.155	2.078	2.117

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Hydrogen influences HDL-associated enzymes and reduces oxidized phospholipids levels in rats fed with a high-fat diet B Liu,Life Sciences,2021
Ginseng-Induced Changes to Blood Vessel Dilation and the Metabolome of Rats HJ Lee,Nutrients,2020

功能：

Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Catalyzes the hydrolysis of 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor or PAF) to 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF). Also catalyzes the transfer of the acetate group from PAF to 1-hexadecanoyl-sn-glycero-3-phosphocholine forming lyso-PAF. Catalyzes the esterification of (24S)-hydroxycholesterol (24(S)OH-C), also known as cerebrosterol to produce 24(S)OH-C monoesters.
亚细胞定位：

Secreted.
蛋白家族：

AB hydrolase superfamily, Lipase family
组织特异性：

Detected in blood plasma (at protein level).
数据库链接：

STRING: 10116.ENSRNOP00000026583

UniGene: Rn.10481

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.