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  4. Rat L-Lactate Dehydrogenase,L-LDH ELISA Kit

Rat L-Lactate Dehydrogenase,L-LDH ELISA Kit

文献引用 (16) 说明书下载
  • 中文名称：
    大鼠L-乳酸脱氢酶(L-LDH)酶联免疫试剂盒
  • 货号：
    CSB-E11324r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO大鼠L-乳酸脱氢酶（L-LDH）酶联免疫检测试剂盒（货号CSB-E11324r），基于双抗体夹心法原理实现定量分析，适用于检测血清、血浆及组织匀浆样本中L-LDH的活性水平。L-LDH是糖酵解途径关键酶，催化丙酮酸与乳酸的可逆转化，其表达水平与细胞能量代谢、组织损伤及病理状态密切相关，常用于评估心肌、肝脏等器官损伤及代谢相关研究。本试剂盒检测灵敏度为39 mU/mL，线性检测范围覆盖39-2500 mU/mL，通过预包被特异性抗体捕获目标蛋白，结合HRP标记检测抗体形成复合物，显色后通过标准曲线计算浓度。该产品可应用于科研场景中药物毒性评估、疾病模型构建、代谢机制研究等领域，实验全程约需4.5小时，提供96孔板规格及标准品、检测抗体、显色液等完整组分，适用于实验室批量样本分析，具有操作便捷、重复性高的特点，为能量代谢研究提供可靠工具。
  • 别名：
    Ldha ELISA Kit; Ldh-1 ELISA Kit; Ldh1 ELISA Kit; L-lactate dehydrogenase A chain ELISA Kit; LDH-A ELISA Kit; EC 1.1.1.27 ELISA Kit; LDH muscle subunit ELISA Kit; LDH-M ELISA Kit
  • 缩写：
    LDHA
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    39 mU/mL-2500 mU/mL
  • 灵敏度：
    9.77 mU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat L-LDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %80-97
    1:2Average %92
    Range %88-105
    1:4Average %97
    Range %92-104
    1:8Average %94
    Range %86-98
  • 回收率：
    The recovery of rat L-LDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9389-100
    EDTA plasma (n=4)9085-95
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/mlOD1OD2AverageCorrected
    25002.754 2.952 2.853 2.679
    12502.218 2.376 2.297 2.123
    6251.534 1.584 1.559 1.385
    312.51.022 1.039 1.031 0.857
    1560.587 0.624 0.606 0.432
    780.412 0.430 0.421 0.247
    390.286 0.279 0.283 0.109
    00.173 0.175 0.174 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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Ldha Proteins

靶点详情

  • 基因功能参考文献：
    1. LDHA is a direct target of miR-34a, which binds to the 3'-UTR region of LDHA mRNA in cardiomyocytes. PMID: 28894025
    2. role of lactate dehydrogenase-A (LDHA), which is a key enzyme for glycolysis, in the proliferation and migration of vascular smooth muscle cells PMID: 28818664
    3. After 30 min of ischemia, the kidney maintains LDH metabolic function in spite of decreased kidney function, whereas the postischemic kidney after 60 min of ischemia show a generally reduced LDH enzyme activity concomitant with a reduced kidney function. PMID: 27654895
    4. The expression of three well-defined downstream targets of hypoxia inducible factor 1 alpha subunit (HIF-1alpha), pyruvate dehydrogenase kinase 1 (PDK1), lactate dehydrogenase A (LDHA) and vascular endothelial growth factor (VEGF), were up-regulated in hippocampus after 28 days of simulated microgravity exposure. PMID: 27988334
    5. aging alters aspects of skeletal muscle isoform shift induced by overload and anabolic steroid administration PMID: 24835193
    6. Lactate dehydrogenase a expression is necessary to sustain rapid angiogenesis of pulmonary microvascular endothelium. PMID: 24086675
    7. lactate dehydrogenase subunit A mRNA stability is regulated by Cyclic AMP and AKAP-mediated targeting of protein kinase A PMID: 15878851
    8. M-LDH released from cardiomyocytes after hypoxia and reoxygenation has a role in protecting the heart from oxidative stress-induced injury through an intracellular signal transduction pathway involving ERK1/2. PMID: 18534967

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    LDH/MDH superfamily, LDH family
  • 数据库链接：

    KEGG: rno:24533

    STRING: 10116.ENSRNOP00000017468

    UniGene: Rn.107896

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |