Rat KI-67 ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

大鼠KI-67酶联免疫试剂盒（CSB-E13175r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的KI-67含量。KI-67 是一种与细胞增殖相关的核抗原。其表达水平与细胞增殖活性密切相关，在正常组织和肿瘤组织中有不同表现。研究中常将其作为评估细胞增殖状态的重要指标，在肿瘤诊断、预后判断等方面有重要价值，用于指导治疗方案的制定。试剂盒检测范围为0.156 ng/mL-10 ng/mL，肿瘤生长评估、组织再生研究、体外培养细胞增殖分析、肿瘤模型研究、药物作用机制探索、基础研究中对细胞动态增殖行为的定量化分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Nifk ELISA Kit; Mki67ipMKI67 FHA domain-interacting nucleolar phosphoprotein ELISA Kit; Nucleolar protein interacting with the FHA domain of pKI-67 ELISA Kit
缩写：

KI-67
Uniprot No.：

Q5RJM0
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.04 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat KI-67 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	83-90
1:2	Average %	93
	Range %	90-96
1:4	Average %	89
	Range %	85-93
1:8	Average %	100
	Range %	95-105

回收率：

The recovery of rat KI-67 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	92	88-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.