Rat Ischemia Modified Albumin,IMA ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠缺血修饰白蛋白（IMA）酶联免疫检测试剂盒（货号CSB-E13620r），采用竞争法定量原理，适用于血清、血浆及组织匀浆样本中IMA含量的精准测定。IMA作为缺血相关病理过程的生物标志物，其水平变化可反映心肌缺血、组织缺氧等生理状态，在心血管疾病、创伤性休克等科研模型中有重要研究价值。该试剂盒检测范围为0.9-60 U/mL，通过预包被特异性抗体实现高灵敏度检测，操作流程包含标准品梯度稀释、样本孵育、显色反应等步骤，实验全程约需4小时。试剂盒提供完整组分包括预包被板、标准品、检测抗体、显色底物及终止液，适用于大鼠心血管疾病机制研究、缺血再灌注损伤模型评估、药物干预效果分析等科研场景，其宽泛的检测范围可满足不同样本基质中低浓度与高浓度IMA的检测需求，组织匀浆的兼容性为离体组织研究提供便利。本产品需配合酶标仪在450nm波长下进行吸光度测定，实验数据通过标准曲线进行定量计算。
缩写：

IMA
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.9 U/mL-60 U/mL
灵敏度：

0.225 U/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IMA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	90-95
1:2	Average %	96
	Range %	90-103
1:4	Average %	102
	Range %	98-106
1:8	Average %	87
	Range %	82-92

回收率：

The recovery of rat IMA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	89	85-93

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.