Rat Interleukin 9,IL-9 ELISA KIT

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO专为大鼠样本设计的白介素9（IL-9）定量检测酶联免疫试剂盒（货号：CSB-E07294r），采用双抗体夹心法原理，可精准检测15.6-1000 pg/mL浓度范围内的IL-9蛋白。IL-9作为Th2细胞分泌的多效性细胞因子，在调控肥大细胞增殖、T细胞分化及过敏性疾病病理机制等科研领域具有重要研究价值。本试剂盒适用于血清、血浆、细胞培养上清及组织匀浆等多种样本类型，实验流程标准化且重复性优异，能够在4.5小时内完成检测。其高灵敏度特性可满足基础科研中低丰度IL-9的定量需求，如炎症模型研究、免疫调控机制探索或药物干预效果评价等场景。试剂盒配套提供预包被捕获抗体的96孔板、生物素标记检测抗体及标准品等完整组分，用户仅需自备常规酶标仪即可开展实验。该产品严格遵循科研用途开发，为研究IL-9在呼吸系统疾病、寄生虫感染或自身免疫疾病中的功能提供可靠工具。
缩写：

IL-9
Uniprot No.：

D4A8I9
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-103
1:2	Average %	92
	Range %	88-96
1:4	Average %	95
	Range %	90-99
1:8	Average %	84
	Range %	80-88

回收率：

The recovery of rat IL-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-95
EDTA plasma (n=4)	98	93-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.