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Rat Interleukin 5,IL-5 ELISA KIT

  • 中文名称:
    大鼠白介素5(IL-5)酶联免疫试剂盒
  • 货号:
    CSB-E07435r
  • 规格:
    96T/48T
  • 价格:
    ¥3200/¥2500
  • 其他:

产品详情

  • 产品描述:
    CUSABIO双抗体夹心法ELISA试剂盒,专为大鼠白介素5(IL-5)的定量检测设计,适用于血清、血浆、细胞培养上清液及组织匀浆样本的分析。IL-5是由Th2细胞分泌的细胞因子,在嗜酸性粒细胞分化与活化中起关键作用,其表达水平与过敏性疾病及寄生虫免疫应答密切相关。试剂盒采用高特异性抗体对,在15.6-1000 pg/mL线性范围内可精准检测目标蛋白,最低检测限达15.6 pg/mL,满足低丰度样本的检测需求。实验操作全程约需4-5小时,包含标准品预稀释步骤,配套提供终止液、洗涤缓冲液等完整试剂组分。该试剂盒适用于基础科研领域中IL-5相关免疫调节机制研究,如构建哮喘动物模型时评估炎症因子水平,或体外药物筛选实验中监测细胞因子分泌动态。样本前处理需注意离心去除颗粒物,组织样本建议按1:5-1:10比例制备匀浆液以确保检测准确性。
  • 别名:
    Il5 ELISA Kit; Il-5 ELISA Kit; Interleukin-5 ELISA Kit; IL-5 ELISA Kit; B-cell growth factor II ELISA Kit; BCGF-II ELISA Kit; Cytotoxic T-lymphocyte inducer ELISA Kit; Eosinophil differentiation factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit
  • 缩写:
  • Uniprot No.:
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围:
    15.6 pg/mL-1000 pg/mL
  • 灵敏度:
    3.9 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %85
    Range %82-91
    1:2Average %92
    Range %83-99
    1:4Average %101
    Range %93-107
    1:8Average %96
    Range %90-104
  • 回收率:
    The recovery of rat IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8885-94
    EDTA plasma (n=4)9490-100
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.407 2.457 2.432 2.268
    5001.768 1.736 1.752 1.588
    2501.065 1.098 1.082 0.918
    1250.704 0.735 0.720 0.556
    62.50.448 0.469 0.459 0.295
    31.20.323 0.300 0.312 0.148
    15.60.221 0.229 0.225 0.061
    00.161 0.167 0.164  
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

靶点详情

  • 功能:
    Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
  • 亚细胞定位:
    Secreted.
  • 蛋白家族:
    IL-5 family
  • 数据库链接: