Rat Interleukin 33(IL-33) ELISA Kit

Rat Interleukin 33(IL-33) ELISA Kit

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中文名称：

大鼠白介素33(IL-33)酶联免疫试剂盒
货号：

CSB-E14077r
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO针对大鼠白介素33（IL-33）的定量检测酶联免疫试剂盒（货号：CSB-E14077r），采用双抗体夹心法原理，可精确检测血清、血浆及组织匀浆样本中IL-33的浓度水平。IL-33作为白介素1家族的重要细胞因子，在Th2型免疫应答、组织炎症及屏障修复中发挥关键调控作用，其表达水平与过敏性疾病、感染反应及组织损伤修复等研究密切相关。试剂盒检测灵敏度为15.6 pg/mL，线性范围覆盖15.6至1000 pg/mL，能够满足低丰度样本的定量需求。实验操作中需使用50μL样本体积，通过预包被特异性抗体的微孔板捕获目标蛋白，经生物素标记检测抗体与链霉亲和素-HRP系统显色，实现高特异性检测。该试剂盒适用于基础科研中炎症机制研究、免疫调控模型分析以及药物干预效果评估等方向，例如在呼吸道炎症模型、自身免疫疾病或创伤修复实验中定量分析IL-33的动态变化。所有组分均经过严格验证，包含标准品、检测抗体及全套实验缓冲液，支持用户快速开展标准化实验。
别名：

Il33Interleukin-33 ELISA kit; IL-33 ELISA kit
缩写：

IL33
Uniprot No.：

Q66H70
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	103
	Range %	98-108
1:2	Average %	96
	Range %	91-101
1:4	Average %	89
	Range %	83-95
1:8	Average %	98
	Range %	93-104

回收率：

The recovery of rat IL-33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-94
EDTA plasma (n=4)	94	90-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.981	2.998	2.990	2.842
500	1.967	1.873	1.920	1.772
250	1.333	1.427	1.380	1.232
125	0.845	0.835	0.840	0.692
62.5	0.565	0.559	0.562	0.414
31.2	0.331	0.348	0.340	0.192
15.6	0.225	0.236	0.231	0.083
0	0.143	0.153	0.148

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Dihydromyricetin treats pulmonary hypertension by modulating CKLF1/CCR5 axis-induced pulmonary vascular cell pyroptosis Q Yan, P Li, S Liu, Y Sun, C Chen, J Long, Y Lin,Biomedicine & Pharmacotherapy,2024
Effect of Src tyrosine kinase on a rat model of asthma M Wu,Exp Ther Med,2022
Lycopene Modulates Placental Health and Fetal Development Under High‐Fat Diet During Pregnancy of Rats S Sun,Molecular Nutrition & Food Research,2021
The possible role of interleukin-33 as a new player in the pathogenesis of contrast-induced nephropathy in diabetic rats Demirtas L.et al,Ren Fail.,2016
Melatonin Attenuates Contrast-Induced Nephropathy in Diabetic Rats: The Role of Interleukin-33 and Oxidative Stress Didem Onk. et al,Mediators of Inflammation,2016

功能：

Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury.; In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation.
基因功能参考文献：
1. The release of IL-33 from necrotic cells might induce autophagy, which can further balance the effects of increased apoptosis secondary to contrast-induced nephropathy in diabetic kidney disease. PMID: 27055881
2. TNF-alpha stimulated IL-33/sST2 and subsequent increase of IL-6 may be the mechanism of IL-33 in acute pancreatitis. PMID: 26927343
3. poly-IC and IL-33 play a role in myelin repair by enhancing expression of myelin genes. PMID: 27022724
4. IL-33 protects against myocardial I/R injury by inhibiting inflammatory responses and myocardial apoptosis. PMID: 26571038
5. These findings indicate that IL-33 might play an important role in the inflammatory response following subarachnoid hemorrhage. PMID: 25417195
6. MRAs play a pivotal role in the Gal-3 and IL-33/ST2 modulation in post-MI cardiac remodeling. PMID: 25458175
7. Induced interleukin-33 expression enhances the tumorigenic activity of rat glioma cells. PMID: 24327583
8. Data indicate IL-33 concentration was increased in the pancreas in duct ligation-induced acute pancreatitis. PMID: 23418608
9. The effects of external application of IL1beta and IL33 on the cardiomyocyte transcriptome, were compared. PMID: 23219998
10. This study examined the expression of IL-33 and the IL-33-mediated regulation of cell functions in pancreatic stellate cells isolated from human and rat pancreas tissues. PMID: 20689058
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亚细胞定位：

Nucleus. Chromosome. Cytoplasm. Cytoplasmic vesicle, secretory vesicle. Secreted.
蛋白家族：

IL-1 family
数据库链接：

KEGG: rno:361749

UniGene: Rn.106849

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.