Rat Interleukin 3,IL-3 ELISA KIT

Rat Interleukin 3,IL-3 ELISA KIT

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中文名称：

大鼠白介素3(IL-3)酶联免疫试剂盒
货号：

CSB-E07436r
规格：

96T/48T
价格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO定量检测大鼠白介素3（IL-3）的酶联免疫试剂盒，IL-3作为重要的造血生长因子，通过促进髓系祖细胞增殖与分化参与免疫调节及炎症反应调控。试剂盒采用双抗体夹心ELISA技术，可精准检测血清、血浆及组织匀浆样本中的IL-3蛋白浓度，检测灵敏度为3.12 pg/mL，线性范围为3.12-200 pg/mL，满足低丰度靶标的定量分析需求。实验流程包含标准品梯度稀释、样本预处理、96孔板孵育及显色反应，操作全程约需4.5小时。该试剂盒适用于科研领域中对IL-3表达水平的研究，例如在造血系统疾病模型、感染性疾病动物实验或免疫相关病理机制探讨中，通过检测血液或组织样本中的IL-3浓度变化，揭示其在炎症反应、细胞分化及免疫调控中的作用规律。试剂盒配套提供预包被抗体、校准品、检测抗体及显色底物，所有组分均经严格质控，确保批次间稳定性与重复性。
别名：

Il3 ELISA Kit; Il-3Interleukin-3 ELISA Kit; IL-3 ELISA Kit; Hematopoietic growth factor ELISA Kit; Mast cell growth factor ELISA Kit; MCGF ELISA Kit; Multipotential colony-stimulating factor ELISA Kit; P-cell-stimulating factor ELISA Kit
缩写：

IL3
Uniprot No.：

P04823
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

3.12 pg/mL-200 pg/mL
灵敏度：

0.78 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	100
	Range %	95-113
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	92-104

回收率：

The recovery of rat IL-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-94
EDTA plasma (n=4)	93	87-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.436	2.276	2.356	2.214
100	1.975	2.014	1.995	1.853
50	1.435	1.372	1.404	1.262
25	0.868	0.913	0.891	0.749
12.5	0.553	0.519	0.536	0.394
6.25	0.387	0.407	0.397	0.255
3.12	0.285	0.276	0.281	0.139
0	0.144	0.140	0.142

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.; This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
亚细胞定位：

Secreted.
蛋白家族：

IL-3 family
组织特异性：

Activated T-cells, mast cells, natural killer cells.
数据库链接：

UniGene: Rn.10652

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.