Rat Interleukin 22(IL-22) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠白介素22（IL-22）酶联免疫检测试剂盒（货号：CSB-E14918r），采用双抗体夹心法定量检测血清、血浆或组织匀浆样本中的IL-22蛋白，检测灵敏度为15.6 pg/mL，线性范围为15.6-1000 pg/mL，适用于科研领域对IL-22表达水平的精准分析。IL-22是IL-10家族细胞因子，主要由Th17细胞和固有淋巴细胞分泌，在黏膜免疫、组织修复及炎症反应中发挥关键作用，其表达水平与肠道炎、皮肤屏障功能等疾病的机制研究密切相关。本试剂盒包含预包被抗体的96孔板、标准品、生物素标记检测抗体及显色系统，通过高特异性抗体配对实现稳定检测，可广泛用于炎症性疾病动物模型（如结肠炎、皮炎或呼吸道感染）的病理机制研究、药物干预效果评价或免疫调控实验。样本兼容性覆盖血清/血浆（系统性表达分析）及组织匀浆（局部病灶检测），实验流程标准化且重复性良好，为科研人员提供可靠的IL-22定量分析工具。
缩写：

IL-22
Uniprot No.：

G3V6X6
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-22 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	83-94
1:2	Average %	99
	Range %	90-102
1:4	Average %	93
	Range %	85-97
1:8	Average %	92
	Range %	84-96

回收率：

The recovery of rat IL-22 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	95-105
EDTA plasma (n=4)	104	92-109

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.