Rat Interleukin 21,IL-21 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO定量检测大鼠血清、血浆及组织匀浆样本中白介素21（IL-21）含量的酶联免疫试剂盒，采用双抗体夹心法原理，检测灵敏度达15.6 pg/mL，线性范围为15.6-1000 pg/mL。IL-21是主要由活化的CD4+ T细胞分泌的细胞因子，通过调节淋巴细胞增殖、分化及抗体生成参与免疫应答，在自身免疫性疾病、肿瘤免疫等领域具有重要研究价值。本试剂盒包含预包被特异性抗体的96孔板及标准品、检测抗体、HRP标记链霉亲和素等核心组分，适用于体外定量分析大鼠外周血或组织样本中IL-21的动态变化，可广泛应用于免疫调节机制研究、炎症性疾病动物模型评估或药物干预后免疫指标变化的科研分析，实验全程仅需4-5小时，标准曲线R²值≥0.99，提供详细操作手册及专业技术支持。
别名：

Il21Interleukin-21 ELISA Kit; IL-21 ELISA Kit
缩写：

IL21
Uniprot No.：

A3QPB9
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	85-96
1:2	Average %	89
	Range %	84-95
1:4	Average %	103
	Range %	99-108
1:8	Average %	99
	Range %	94-105

回收率：

The recovery of rat IL-21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-101
EDTA plasma (n=4)	92	88-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation.
亚细胞定位：

Secreted.
蛋白家族：

IL-15/IL-21 family
数据库链接：

KEGG: rno:365769

STRING: 10116.ENSRNOP00000023348

UniGene: Rn.214949