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  4. Rat Interleukin 2,IL-2 ELISA kit

Rat Interleukin 2,IL-2 ELISA kit

文献引用 (13) 说明书下载
  • 中文名称：
    大鼠白介素2(IL-2)酶联免疫试剂盒
  • 货号：
    CSB-E04628r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO大鼠白介素2（IL-2）酶联免疫检测试剂盒（货号：CSB-E04628r），采用双抗体夹心法定量检测血清、尿液及组织匀浆样本中IL-2的表达水平。IL-2作为T淋巴细胞分泌的关键细胞因子，在免疫调节中发挥重要作用，参与T细胞增殖分化、NK细胞活化及免疫耐受调控等生物学过程。试剂盒检测灵敏度为6.25 pg/mL，线性检测范围为6.25-400 pg/mL，可精准测定不同浓度梯度的目标蛋白。该产品适用于科研实验中评估大鼠免疫激活状态，例如研究炎症性疾病模型（如自身免疫疾病或感染模型）中细胞因子网络的变化规律，亦可应用于药物研发中免疫调节剂的效果评价，或通过分析组织匀浆样本探究局部免疫微环境的IL-2分泌特征。操作流程包含标准品梯度稀释、样本孵育、酶标抗体结合及显色反应等步骤，配套试剂组分完整，支持科研人员高效完成实验数据采集。
  • 别名：
    Il2 ELISA kit; Il-2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit
  • 缩写：
    IL2
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, urine, tissue homogenates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %87-98
    1:2Average %98
    Range %91-104
    1:4Average %89
    Range %84-99
    1:8Average %96
    Range %92-100
  • 回收率：
    The recovery of rat IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9085-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.489 2.478 2.484 2.289
    2001.954 1.896 1.925 1.730
    1001.292 1.273 1.283 1.088
    500.797 0.768 0.783 0.588
    250.503 0.489 0.496 0.301
    12.50.364 0.356 0.360 0.165
    6.250.276 0.258 0.267 0.072
    00.198 0.192 0.195 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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Il2 Proteins

靶点详情

  • 功能：
    Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
  • 基因功能参考文献：
    1. Data indicate that C-rel binding on the interleukin-2 (IL-2) promoter region declined in the aged CD4+T cells. PMID: 24853588
    2. our findings indicate a generic and robust stress-induced reduction in plasma IL-12 PMID: 22659252
    3. Effects of systemic IL-2 on neurotransmission in several cortical areas is measured by in-vivo microdialysis; IL-2 has dose-dependent effects on depressive-related behavior after delayed but not acute testing, but no effects on anxiety-like behavior. PMID: 21726905
    4. The purpose of this study was to evaluate the effect of exhaustive exercise on proteins associated with muscle damage and regeneration, including IL-2, IL-4 and MyoD in muscle and adipose tissue. PMID: 21668966
    5. IL-2 neuroprotective action upon retinal ganglion cells in vitro is mediated by Janus kinase and extracellular signal-regulated kinase 1/2 activation. PMID: 21262542
    6. In a living cell imaging study, treatment with IL-2 at different concentrations during development of cultured hippocampal neurons induces major changes in dendritic branches, filopodia mobility, and spine density. PMID: 20225203
    7. The pIL-2-shRNA interference plasmid could decrease proinflammatory cytokine production of T lymphocytes and protect hepatocytes from apoptosis in allografts. PMID: 19589546
    8. IL-2 may participate in the immune rejection in allogeneic liver transplantation. PMID: 18471351
    9. effects of IL-2 on contraction and the [Ca2+]i transient of ventricular myocytes are mediated via the cardiac kappa opioid receptor. PMID: 11907831
    10. IL-1alpha and IL-2 produce an increase of glucose metabolism in uteri isolated from underfed rats PMID: 12878449
    11. Analysis of murine CD28 mutants reveals a correlation between translocation to lipid rafts and costimulation of IL-2 production. PMID: 15280538
    12. These results provide new evidence indicating that cytokine mRNA in the brain can be related to elevated plus-maze behavior and that this relationship is site (prefrontal cortex, striatum)- and cytokine mRNA-specific (IL-2). PMID: 15950774
    13. hypothalamic IL2 is decreased after intranasal administration of Epithalon PMID: 17152351
    14. stress-induced increase in serum IL-6 level and decrease in IL-2 level PMID: 17433709
    15. Cell activation in hypothalamus correlated with decreases in the quantity of IL-2-positive cells. PMID: 18264770
    16. Data suggest that estradiol effects on T cell IFN-gamma abd IL-2 production afer trauma-hemorrhage are mediated via non-genomic pathway, and which is in turn mediated via ERK, p38, and JNK pathways. PMID: 18343154
    17. Data show that inescapable and escapable stressors have different effects on behavior in the modified active avoidance test and on IL-2 levels in brain areas that are known to be involved in emotional processes. PMID: 18628674
    18. CD4+CD25+ T cells alloactivated ex vivo by IL-2 or IL-4 become potent alloantigen-specific inhibitors of rejection with different phenotypes, suggesting separate pathways of activation by Th1 and Th2 responses. PMID: 18827184

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    IL-2 family
  • 数据库链接：

    KEGG: rno:116562

    STRING: 10116.ENSRNOP00000023327

    UniGene: Rn.9871

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