Rat Interleukin 1β,IL-1β ELISA Kit

Rat Interleukin 1β,IL-1β ELISA Kit

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中文名称：

大鼠白介素1β (IL-1β)酶联免疫试剂盒
货号：

CSB-E08055r-IS
规格：

96T/48T
价格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO双抗体夹心法大鼠白介素1β（IL-1β）定量检测试剂盒（货号：CSB-E08055r-IS），适用于血清、血浆及组织匀浆样本的科研分析。IL-1β作为关键促炎细胞因子，在免疫应答、炎症反应及组织损伤修复中发挥核心作用，其动态变化常用于评估感染性疾病、自身免疫病及神经退行性病变等研究模型。试剂盒采用高特异性配对抗体，实现31.25-2000 pg/mL的宽线性检测范围，最低检测限达15.6 pg/mL，可精准捕捉生物样本中IL-1β的微量表达差异。实验操作配备预包被96孔板及标准化显色系统，3.5小时内即可完成检测流程，兼容酶标仪450nm波长读数。该试剂盒广泛用于炎症机制研究、药物抗炎效果评价、免疫调控实验及动物疾病模型构建，特别适用于脑、肝脏等组织匀浆中细胞因子释放的动态监测。配套提供标准品、生物素化检测抗体及终止液等完整组分，实验结果需通过标准曲线进行定量计算。
别名：

Il1bInterleukin-1 beta ELISA kit; IL-1 beta ELISA kit
缩写：

IL1B
Uniprot No.：

Q63264
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

15.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	91
	Range %	91-105
1:2	Average %	102
	Range %	95-109
1:4	Average %	99
	Range %	92-105
1:8	Average %	93
	Range %	86-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

2000

2.826

2.634

2.730

2.569

1000

2.045

1.967

2.006

1.845

500

1.349

1.328

1.339

1.177

250

0.818

0.829

0.824

0.662

125

0.474

0.491

0.482

0.321

62.5

0.344

0.353

0.349

0.187

31.25

0.238

0.244

0.241

0.080

0.157

0.165

0.161

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
亚细胞定位：

Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
蛋白家族：

IL-1 family
数据库链接：

STRING: 10116.ENSRNOP00000006308

UniGene: Rn.9869

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.