Rat Interleukin-17F(IL17F) ELISA kit

中文名称：

大鼠白介素17F(IL17F)酶联免疫试剂盒
货号：

CSB-EL011601RA
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO针对大鼠白介素17F（IL17F）定量检测的酶联免疫试剂盒，采用双抗体夹心法原理，适用于血清、血浆及组织匀浆样本的精准分析。IL-17F是IL-17细胞因子家族成员之一，在炎症反应调控、宿主防御及自身免疫性疾病中发挥重要作用，其表达水平与多种病理过程密切相关。该试剂盒检测灵敏度为15.6 pg/mL，线性检测范围覆盖15.6-1000 pg/mL，可满足低浓度样本的检测需求。实验操作基于预包被特异性抗体的微孔板，通过生物素标记检测抗体与链霉亲和素-HRP系统实现信号放大，配合标准品浓度梯度建立标准曲线进行定量分析。本试剂盒适用于科研领域中对大鼠模型中IL-17F表达水平的动态监测，如炎症性疾病机制研究、免疫调节实验或药物干预效果评估等场景，为探索IL-17F在免疫系统中的功能提供可靠工具。试剂盒包含标准品、预混检测溶液及必要反应组分，经严格验证确保批间稳定性，实验流程优化后可在常规实验室条件下完成。
别名：

Il17fInterleukin-17F ELISA Kit; IL-17F ELISA Kit
缩写：

IL17F
Uniprot No.：

Q5BJ95
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL17F in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-99
1:2	Average %	83
	Range %	80-86
1:4	Average %	95
	Range %	80-90
1:8	Average %	102
	Range %	98-106

回收率：

The recovery of rat IL17F spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	83-90
EDTA plasma (n=4)	104	100-108

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.806	2.769	2.788	2.693
500	2.304	2.401	2.353	2.258
250	1.535	1.605	1.570	1.475
125	0.680	0.698	0.689	0.594
62.5	0.343	0.351	0.347	0.252
31.2	0.202	0.199	0.201	0.106
15.6	0.127	0.129	0.128	0.033
0	0.095	0.094	0.095

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

引用文献

Identification and immunogenicity of microneme protein 2 (EbMIC2) of Eimeria brunetti Hoan TD. et al,Exp Parasitol,2015

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功能：

Effector cytokine of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity. IL17A-IL17F signals via IL17RA-IL17RC heterodimeric receptor complex, triggering homotypic interaction of IL17RA and IL17RC chains with TRAF3IP2 adapter through SEFIR domains. This leads to downstream TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways ultimately resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides and matrix metalloproteinases, with potential strong immune inflammation. IL17A-IL17F is primarily involved in host defense against extracellular bacteria and fungi by inducing neutrophilic inflammation. As signature effector cytokine of T-helper 17 cells (Th17), primarily induces neutrophil activation and recruitment at infection and inflammatory sites. Stimulates the production of antimicrobial beta-defensins DEFB1, DEFB103A, and DEFB104A by mucosal epithelial cells, limiting the entry of microbes through the epithelial barriers. IL17F homodimer can signal via IL17RC homodimeric receptor complex, triggering downstream activation of TRAF6 and NF-kappa-B signaling pathway. Via IL17RC induces transcriptional activation of IL33, a potent cytokine that stimulates group 2 innate lymphoid cells and adaptive T-helper 2 cells involved in pulmonary allergic response to fungi. Likely via IL17RC, promotes sympathetic innervation of peripheral organs by coordinating the communication between gamma-delta T cells and parenchymal cells. Stimulates sympathetic innervation of thermogenic adipose tissue by driving TGFB1 expression. Regulates the composition of intestinal microbiota and immune tolerance by inducing antimicrobial proteins that specifically control the growth of commensal Firmicutes and Bacteroidetes.
基因功能参考文献：
1. Increased RORgammat and IL-17F levels contribute to Spontaneously Hypertensive Rat hypertension and might be therapeutic targets PMID: 28093217
2. important mediator of increased endothelial permeability PMID: 19577259
亚细胞定位：

Secreted.
蛋白家族：

IL-17 family
数据库链接：

KEGG: rno:301291

STRING: 10116.ENSRNOP00000016717

UniGene: Rn.34022

Rat Interleukin-17F(IL17F) ELISA kit

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