Rat Interleukin 16,IL-16 ELISA KIT

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

大鼠白介素16(IL-16)酶联免疫试剂盒（CSB-E07452r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL16含量。IL16作为一个靶点，其背景上是具有免疫调节功能的细胞因子。在研究机制方面，它能与CD4受体结合，参与T淋巴细胞等的活化、增殖与迁移过程，在免疫相关疾病研究中受关注，为疾病治疗策略开发提供潜在方向。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于大鼠炎症机制研究、免疫相关疾病模型评估及药物干预效果分析等科研领域；可满足细胞因子网络研究、免疫微环境分析等实验需求，尤其适用于神经科学、免疫学及药理学领域的体外样本检测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

IL-16
Uniprot No.：

G5BEZ3
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-96
1:2	Average %	98
	Range %	94-102
1:4	Average %	103
	Range %	98-107
1:8	Average %	89
	Range %	84-96

回收率：

The recovery of rat IL-16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	88	82-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.