Rat Interleukin 15,IL-15 ELISA KIT

Rat Interleukin 15,IL-15 ELISA KIT

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中文名称：

大鼠白介素15(IL-15)酶联免疫试剂盒
货号：

CSB-E07453r
规格：

96T/48T
价格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO高灵敏大鼠白介素15（IL-15）酶联免疫检测试剂盒（货号：CSB-E07453r），采用双抗体夹心法定量分析血清、血浆、组织匀浆及细胞裂解液中的IL-15水平。IL-15是一种多效性细胞因子，在调节天然免疫和适应性免疫应答中发挥重要作用，尤其在T细胞、自然杀伤细胞（NK细胞）的增殖与活化中具有关键调控功能。本试剂盒检测范围为1.56-100 pg/mL，可精准检测微量样本中IL-15的表达动态，适用于炎症反应、免疫性疾病模型构建、药物干预效果评估等科研场景。实验通过预包被特异性捕获抗体与标记检测抗体的协同作用，结合显色反应实现目标蛋白的定量分析，具有操作简便、重复性好的特点。该试剂盒兼容多种生物样本类型，可为免疫调控机制研究、感染性疾病模型分析及生物标志物筛选提供可靠工具，满足基础科研与转化医学研究中对IL-15动态监测的需求。
别名：

Il15 ELISA Kit; Interleukin-15 ELISA Kit; IL-15 ELISA Kit
缩写：

IL15
Uniprot No.：

P97604
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

1.56 pg/mL-100 pg/mL
灵敏度：

0.39 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-101
1:2	Average %	93
	Range %	87-99
1:4	Average %	87
	Range %	82-94
1:8	Average %	100
	Range %	95-104

回收率：

The recovery of rat IL-15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-101
EDTA plasma (n=4)	93	87-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
100	2.511	2.567	2.539	2.383
50	1.773	1.783	1.778	1.622
25	1.106	1.154	1.130	0.974
12.5	0.704	0.725	0.715	0.559
6.25	0.415	0.431	0.423	0.267
3.12	0.287	0.297	0.292	0.136
1.56	0.214	0.231	0.223	0.067
0	0.155	0.157	0.156

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Amentoflavone Mitigates Cyclophosphamide-Induced Pulmonary Toxicity: Involvement of-SIRT-1/Nrf2/Keap1 Axis, JAK-2/STAT-3 Signaling, and Apoptosis MF Balaha,Medicina,2023
Interleukin-15 derived from Guanylin?GC-C-expressing macrophages inhibits fatty acid synthase in adipocytes Akieda-Asai S.et al,Peptides,2017
Effects of acute and chronic resistance exercise training on IL-15 expression in rat skeletal muscle. Zanchi NE et al,Eur J Appl Physio,2010

功能：

Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA. In neutrophils, stimulates phagocytosis probably by signaling through the IL15 receptor, composed of the subunits IL15RA, IL2RB and IL2RG, which results in kinase SYK activation.
基因功能参考文献：
1. study indicates that training, skeletal muscle phenotype, and metabolic status all influence the temporal pattern of post-exercise changes in IL-15 expression. PMID: 25522723
2. found that in diabetic rats, resistance training increased muscle and serum IL-15 levels PMID: 24006180
3. results suggested that IL-15-Akt signaling harbored the complex signals for muscle-T cell interaction, the regulatory networks have significant potential for the restriction on IL-1beta inflammatory signaling PMID: 23017227
4. IL-15 is related to organ dysfunction during severe acute pancreatitis, and it functions as a protective factor against the organ injuries. PMID: 20332642
5. The results of this study suggest that the IL-15 signal transduction pathway in neural cells is similar to that in immune cells. PMID: 19747902
6. IL-15 might antagonize some cytokine mediated cell death in islet cells. PMID: 12880684
7. IL-15 produced by metastatic colon carcinoma cells can induce hyperplasia in the mucosa adjacent to colon cancer, thus contributing to angiogenesis and progression of the disease PMID: 14581351
8. Cocultivation of lymphocytes with pancreatic stellate cells and IL-15 inhibited spontaneous lymphocyte apoptosis, and this effect was reduced by an anti-IL-15 antibody PMID: 16002563
9. the decrease of CORT secretion by IL-15 is in part because of (i) the decrease of adenylate cyclase activity and cAMP production and (ii) the inhibition of 3beta-hydroxysteroid dehydrogenase and 11beta-hydroxylase activities in rat ZFR cells. PMID: 16405651
10. Increases in IL-15 mRNA in response to atrophic stimuli may be an attempt to counteract muscle mass loss in skeletal muscles of old animals. PMID: 17135303
11. IL-15 might have an inhibitory effect on the physiologic vascular remodeling processes in closing the ductus arteriosus. PMID: 17667861
12. The changes observed in BAT suggest that IL-15 could be implicated in lipid consumption in this tissue by regulating lipid oxidation and probably thermogenesis, processes mediated by UCP1 and UCP3 and PPARdelta and PPARalpha. PMID: 18239634
13. Preservation of IL-15 signaling by calorie restriction may therefore represent a further mechanism contributing to the anti-aging effect of this dietary intervention in skeletal muscle PMID: 19396981
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亚细胞定位：

Secreted.
蛋白家族：

IL-15/IL-21 family
数据库链接：

KEGG: rno:25670

UniGene: Rn.2490

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.