Rat Interleukin 12,IL-12/P40 ELISA KIT

Rat Interleukin 12,IL-12/P40 ELISA KIT

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中文名称：

大鼠白介素12(IL-12/P40)酶联免疫试剂盒
货号：

CSB-E07362r
规格：

96T/48T
价格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO大鼠白介素12（IL-12/P40）酶联免疫检测试剂盒（货号CSB-E07362r），采用双抗体夹心法原理实现精准定量分析。IL-12是由巨噬细胞和树突状细胞分泌的重要细胞因子，其P40亚基作为IL-12和IL-23的共同结构单元，在调节Th1型免疫应答和抗肿瘤免疫中发挥关键作用。该试剂盒可检测血清、血浆及组织匀浆样本，线性检测范围覆盖31.25-2000 pg/mL，灵敏度达到15.6 pg/mL，实验全程约需4.5小时完成。配备预包被捕获抗体、生物素标记检测抗体及标准品体系，适用于科研领域探究感染性疾病、自身免疫疾病或肿瘤微环境中IL-12/P40的动态变化，为免疫调控机制研究、动物疾病模型评估或药物干预效果分析提供可靠工具。试剂盒严格通过交叉反应验证，确保对大鼠样本的特异性识别，实验数据可通过酶标仪在450nm波长下读取，配套提供详细操作指南及数据分析支持。
别名：

N/A
缩写：

IL-12/P40
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	96-106
1:2	Average %	94
	Range %	90-98
1:4	Average %	95
	Range %	90-99
1:8	Average %	89
	Range %	82-94

回收率：

The recovery of rat IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-104
EDTA plasma (n=4)	103	97-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.329	2.228	2.279	2.077
1000	1.748	1.647	1.698	1.496
500	1.152	1.131	1.142	0.940
250	0.794	0.783	0.789	0.587
125	0.506	0.495	0.501	0.299
62.5	0.415	0.404	0.410	0.208
31.25	0.308	0.303	0.306	0.104
0	0.202	0.201	0.202

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Immune-Enhancing Activity of Vitis coignetiae Extract via Increasing Cytokine and Natural Killer Cell Activity in Splenocytes and Cyclophosphamide-Induced Immunosuppressed Rats YM Park,Journal of Food Biochemistry,2024
Early-life bisphenol A exposure causes neuronal pyroptosis in juvenile and adult male rats through the NF-κB/IL-1β/NLRP3/caspase-1 signaling pathway: exploration of age and dose as effective covariates using an in vivo and in silico modeling approach AS Al,Molecular and cellular biochemistry,2024
Immune-Enhancing Activity of Vitis coignetiae Extract via Increasing Cytokine and Natural Killer Cell Activity in Splenocytes and Cyclophosphamide-Induced Immunosuppressed Rats YM Park,/,2024
Extracellular vesicles from Trichinella spiralis: Proteomic analysis and protective immunity X Gao,PLoS neglected tropical diseases,2022
Immunomodulatory Effect of Fermented Vinegar on Cyclophosphamide-induced Immunosuppression Model HS Hwa,Journal of Food and Nutrition Research,2021
A Novel Combination Therapy Using Rosuvastatin and Lactobacillus Combats Dextran Sodium Sulfate-Induced Colitis in High-Fat Diet-Fed Rats by Targeting the TXNIP/NLRP3 Interaction and Influencing Gut Microbiome Composition Sameh Saber,Pharmaceuticals,2021
The immunological and protective role of Baicalin in male rats treated with chemotherapy (Gemcitabine) Maytham T. Qasim, et al,Nanoscience and Nanotechnology Letters,2019
High-dose dexamethasone induced LPS-stimulated rat alveolar macrophages apoptosis Zeng S.et al,Drug Des Devel Ther,2017
Immunostimulative effects of Cyperus rotundus, Alpinia calcarata, Solanum surattense, Clerodendrum infortunatum and Croton laccifer extracts combination on ? Ediriweera P. S. et al,Journal of Experimental and Integrative Medicine,2015

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.