Rat Interleukin 11,IL-11 ELISA KIT

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

大鼠白介素11（IL-11）酶联免疫试剂盒（货号：CSB-E07358r）采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中的IL-11蛋白水平，其检测灵敏度达15.6 pg/mL，线性范围覆盖15.6至1000 pg/mL，适用于精准分析低浓度样本。IL-11作为多功能细胞因子，在造血调控、免疫应答及组织修复中发挥重要作用，其表达水平变化与炎症反应、纤维化疾病及肿瘤微环境调控等研究密切相关。本试剂盒通过特异性捕获抗体与检测抗体的协同作用实现高灵敏度检测，实验流程兼容常规酶标仪分析，操作便捷且重复性优异。科研人员可借助该产品探究IL-11在炎症性疾病模型、再生医学研究或药物干预实验中的动态变化，为揭示其生物学功能及信号通路机制提供可靠数据支持。该试剂盒严格适用于体外科研用途，不涉及任何临床诊断相关应用。
别名：

Il11 ELISA Kit; Interleukin-11 ELISA Kit; IL-11 ELISA Kit
缩写：

IL11
Uniprot No.：

Q99MF5
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-94
1:2	Average %	85
	Range %	80-90
1:4	Average %	92
	Range %	86-101
1:8	Average %	95
	Range %	89-102

回收率：

The recovery of rat IL-11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	80-105
EDTA plasma (n=4)	98	90-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. Also promotes the proliferation of hepatocytes in response to liver damage. Binding to its receptor formed by IL6ST and IL11RA activates a signaling cascade that promotes cell proliferation. Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3. The interaction with the membrane-bound IL11RA and IL6ST stimulates 'classic signaling', whereas the binding of IL11 and soluble IL11RA to IL6ST stimulates 'trans-signaling'.
亚细胞定位：

Secreted.
蛋白家族：

IL-6 superfamily
数据库链接：

STRING: 10116.ENSRNOP00000023489

UniGene: Rn.198483