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  4. Rat Interleukin 1 receptor antagonist, IL-1ra ELISA kit

Rat Interleukin 1 receptor antagonist, IL-1ra ELISA kit

文献引用 (2) 说明书下载
  • 中文名称：
    大鼠白介素1受体拮抗剂(IL1Ra)酶联免疫试剂盒
  • 货号：
    CSB-E10397r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    大鼠白介素1受体拮抗剂(IL1Ra)酶联免疫试剂盒（CSB-E10397r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL1RN含量。IL1RN是重要靶点。它属于白细胞介素-1受体拮抗剂，可竞争结合IL-1受体。背景上，其失衡与多种炎症性疾病相关。研究机制方面，主要是通过阻断IL-1信号通路，抑制炎症反应，在炎症疾病治疗研究里具有重要价值。试剂盒检测范围为125 pg/mL-8000 pg/mL，适用于科研领域中对炎症机制、自身免疫疾病模型或药物干预效果的动态研究。为探索IL - 1信号通路调控、评估实验动物体内抗炎反应强度提供了可靠工具，广泛应用于基础医学、药理学及生物标志物研究等领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Il1rn ELISA Kit; Il-1raInterleukin-1 receptor antagonist protein ELISA Kit; IL-1RN ELISA Kit; IL-1ra ELISA Kit; IRAP ELISA Kit; IL1 inhibitor ELISA Kit
  • 缩写：
    IL1RN
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    125 pg/mL-8000 pg/mL
  • 灵敏度：
    31.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-1ra in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %102
    Range %98-105
    1:2Average %97
    Range %92-102
    1:4Average %96
    Range %90-101
    1:8Average %93
    Range %88-99
  • 回收率：
    The recovery of rat IL-1ra spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9792-104
    EDTA plasma (n=4)8982-95
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    80002.259 2.269 2.264 2.148
    40001.554 1.597 1.576 1.460
    20001.040 1.068 1.054 0.938
    10000.625 0.672 0.649 0.533
    5000.398 0.371 0.385 0.269
    2500.279 0.274 0.277 0.161
    1250.181 0.189 0.185 0.069
    00.114 0.118 0.116 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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Il1rn Proteins

靶点详情

  • 功能：
    Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity.
  • 基因功能参考文献：
    1. IL-1beta is the key trigger of neuroinflammation and that IL-1ra prevents the neuroinflammation entering the chronic state PMID: 24621600
    2. Interleukin-1beta and interleukin-1 receptor antagonist appear in grey matter additionally to white matter during experimental multiple sclerosis PMID: 24376764
    3. Gene expression of the natural IL-1 receptor antagonist IL-1ra and IL-18 (but not TNFalpha) is increased in the ipsilateral hippocampus during long-term potentiation. PMID: 23747799
    4. the IL-1ra gene could be transferred and expressed in corneal tissue efficiently. This may be a novel technique for gene transfection to the cornea in situ. PMID: 22827713
    5. Energy-sensing factors coactivator peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1alpha) and AMP-activated protein kinase control expression of inflammatory mediators in liver: induction of interleukin 1 receptor antagonist. PMID: 22117073
    6. IL1Ra has a role in reducing islet endothelial activation and oxidative stress gene expression in the GK diabetic rat PMID: 19742300
    7. IL-1ra, even when applied for only 4 h, reduces neuronal cell death and the number of microglial cells after excitotoxic damage PMID: 15932594
    8. rhIL-1ra may protect renal injury after asphyxia via inhibiting intrarenal inflammatory response. PMID: 16259926
    9. IL-1ra is a modulator of gender differences in the febrile response to lipopolysaccharides. PMID: 17138728
    10. physical training has an effect on IL-1beta, IL-6 and IL-1ra concentrations in various brain areas of the rat PMID: 18299269
    11. Estrogen-induced protection is associated with a decrease in IL-1beta and an increase in IL-1ra expression in the ischemic hippocampus which suggests that modulation of IL-1beta/IL-1ra might be a part of anti-inflammatory effects of estrogen. PMID: 18455872
    12. A pro-inflammatory orientation in the interleukin-1 beta/interleukin-1 receptor antagonist balance might play a role in the initiation of perinatal brain damages. PMID: 18511291

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    IL-1 family
  • 数据库链接：

    KEGG: rno:60582

    STRING: 10116.ENSRNOP00000007949

    UniGene: Rn.162640

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |