Rat Interferon β,IFN-β/IFNB ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠β干扰素（IFN-β/IFNB）定量检测试剂盒（货号：CSB-E04845r），采用双抗体夹心ELISA技术，可精准检测血清、血浆及组织匀浆样本中IFN-β的浓度水平，检测范围为15.6-1000 pg/mL，灵敏度达7.8 pg/mL。试剂盒基于特异性抗体对目标蛋白的捕获与标记检测，配套标准品、预包被96孔板及检测所需全套试剂，适用于科研实验中IFN-β的动态监测。IFN-β作为I型干扰素家族成员，在抗病毒免疫应答及免疫调节中发挥核心作用，其表达水平与病毒感染、炎症反应及自身免疫性疾病机制密切相关。本试剂盒可为病毒感染模型构建、免疫调节机制研究及药物干预效果评估提供定量数据支持，尤其适用于动物实验中对IFN-β分泌水平的分析，如中枢神经系统炎症、病毒性肝炎或自身免疫性疾病等研究场景。试剂盒操作流程标准化，重复性良好，满足实验室对生物标志物检测的高通量需求。
别名：

Ifnb1 ELISA Kit; IfnbInterferon beta ELISA Kit; IFN-beta ELISA Kit
缩写：

IFNB1
Uniprot No.：

P70499
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig LIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-102
1:2	Average %	104
	Range %	98-110
1:4	Average %	90
	Range %	85-94
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of pig LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	87	80-91

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Has antiviral, antibacterial and anticancer activities.
基因功能参考文献：
1. results provided evidence that delivery of IL-18 and IFN-beta by BMSCs may be an excellent and promising approach to develop an effective treatment protocol for glioma therapy. PMID: 26252165
2. IFN-gamma is able to activate rat intestinal mucous microvascular endothelial cells, thereby leading to upregulated expressions of IFN-alpha/beta. PMID: 20214528
3. These findings validate IFN-beta as a therapeutic molecule for intraarticular gene therapy of arthritis. PMID: 16984225
4. This study presents novel effects of IFN-beta in the central nervous system and begins to unravel the mechanism behind the known excitability changes in neurons. PMID: 19541371
亚细胞定位：

Secreted.
蛋白家族：

Alpha/beta interferon family
数据库链接：

KEGG: rno:24481

STRING: 10116.ENSRNOP00000008225

UniGene: Rn.138105