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  4. Rat Insulin-like growth factor binding protein 1,IGFBP-1 ELISA Kit

Rat Insulin-like growth factor binding protein 1,IGFBP-1 ELISA Kit

文献引用 (1) 说明书下载
  • 中文名称：
    大鼠胰岛素样生长因子结合蛋白1(IGFBP-1)酶联免疫试剂盒
  • 货号：
    CSB-E07349r
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    大鼠胰岛素样生长因子结合蛋白1(IGFBP-1)酶联免疫试剂盒（CSB-E07349r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IGFBP1含量。IGFBP1即胰岛素样生长因子结合蛋白1，它在调节细胞生长、分化和代谢等方面有重要作用。作为一种靶点，研究机制聚焦于其与胰岛素样生长因子的相互作用，以及对下游信号通路影响，以探寻其在肿瘤、代谢疾病等病理过程中的潜在干预方式。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于基础科研领域，可用于研究IGFBP-1在大鼠模型中的表达调控机制，探索其在代谢性疾病、生长发育异常或组织损伤修复中的潜在作用，亦可用于体外药物干预实验或疾病模型构建中相关生物标志物的定量分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Igfbp1 ELISA Kit; Igfbp-1 ELISA Kit; Insulin-like growth factor-binding protein 1 ELISA Kit; IBP-1 ELISA Kit; IGF-binding protein 1 ELISA Kit; IGFBP-1 ELISA Kit
  • 缩写：
    IGFBP1
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IGFBP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 87  
    Range % 80-94  
    1:200 Average % 97  
    Range % 91-105  
    1:400 Average % 88  
    Range % 81-95  
    1:800 Average % 100  
    Range % 95-94  
  • 回收率：
    The recovery of rat IGFBP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-99  
    EDTA plasma (n=4) 92 87-97  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.142 2.287 2.215 2.030  
    500 1.882 1.897 1.890 1.705  
    250 1.297 1.324 1.311 1.126  
    125 0.803 0.815 0.809 0.624  
    62.5 0.492 0.506 0.499 0.314  
    31.2 0.360 0.387 0.374 0.189  
    15.6 0.273 0.288 0.281 0.096  
    0 0.184 0.186 0.185 　  
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

产品评价

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Igfbp1 Proteins

靶点详情

  • 功能：
    IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.
  • 基因功能参考文献：
    1. In simulated sleep apnea hypopnea syndrome, intermittent hypoxia in young rats does not cause physical growth retardation, but serum IGF-1 and IGFBP-3 levels decreased with the increase of hypoxia and decline of oxygen saturation. PMID: 22805023
    2. up-regulation of IGFBP-1 in oligodendrocytes in multiple sclerosis may serve two functions: (i) regulate IGF-1 actions, (ii) exert IGF-independent effects through its RGD sequence PMID: 20345750
    3. Insulin regulation of insulin-like growth factor-binding protein-1 gene expression PMID: 11784721
    4. regulation of expression by insulin is impaired by presence of hydrogen peroxide PMID: 11942857
    5. IGFBP-1 and IGFBP-3 are present in phosphorylated form in fasted rat skin, which induced inhibition of collagen biosynthesis in cultured fibroblasts PMID: 12670795
    6. IGFBP-1 released during the early steps of liver tissue damage and repair may interact with liver cells and potentiate the sensitivity of IGF-I to mitogenic signals. PMID: 15070850
    7. In this report we demonstrate that in H4IIE-C3 cells, four distinct classes of GSK-3 inhibitor mimic the effect of insulin on a third thymine-rich insulin response element-containing gene, IGFBP-1. PMID: 15350195
    8. Iron, radical oxygen species, and HIF-2 and -3 as well as prolyl hydroxylase pathways play important roles in mediating effects of hypoxia on IGFBP-1 gene expression in liver. PMID: 16166214
    9. insulin inhibition of IGFBP-1 mRNA levels can occur in the absence of the phosphorylation of Foxo1/Foxo3, whereas activation of the mTOR pathway is both necessary and sufficient PMID: 16455781
    10. Activation of the mTOR pathway, without activation of its upstream regulator PI 3-kinase, reduces IGFBP1 expression. PMID: 17032741
    11. Age-related oxidative stress underlies the upregulated JNK activation and IGFBP-1 expression. PMID: 17645865

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  • 亚细胞定位：
    Secreted.
  • 数据库链接：

    KEGG: rno:25685

    STRING: 10116.ENSRNOP00000011338

    UniGene: Rn.34026

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |