Rat IL-1 Receptor Like 1(IL1RL1)ELISA Kit

Rat IL-1 Receptor Like 1(IL1RL1)ELISA Kit

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中文名称：

大鼠白介素1受体样1(IL1RL1)酶联免疫试剂盒
货号：

CSB-E14896r
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO针对大鼠白介素1受体样1（IL1RL1）的定量检测试剂盒（货号：CSB-E14896r），采用双抗体夹心ELISA技术，可精准检测血清、血浆、细胞培养上清及组织匀浆样本中IL1RL1的蛋白含量。IL1RL1作为白介素1受体家族成员，广泛参与炎症反应调控、组织修复及免疫稳态维持等生物学过程，其表达水平与哮喘、心肌纤维化等病理机制密切相关。本试剂盒检测灵敏度高，线性范围为15.6 pg/mL至1000 pg/mL，通过预包被特异性捕获抗体与标记检测抗体的协同作用，实现对目标蛋白的特异性识别与定量分析。适用于科研领域中对IL1RL1表达动态的探索，例如炎症性疾病动物模型的机制研究、药物干预后生物标志物水平变化监测，或心血管疾病相关信号通路的功能解析。实验操作简便，配套标准品及优化缓冲液体系可确保数据可靠性，为研究者提供高效、稳定的检测工具。
别名：

Il1rl1 ELISA Kit; Fit1 ELISA Kit; Interleukin-1 receptor-like 1 ELISA Kit; EC 3.2.2.6 ELISA Kit; Fit-1 ELISA Kit; Fos-responsive gene 1 protein ELISA Kit
缩写：

IL1RL1
Uniprot No.：

Q62611
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL1RL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	97
	Range %	91-104
1:4	Average %	96
	Range %	91-101
1:8	Average %	92
	Range %	86-96

回收率：

The recovery of rat IL1RL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-100
EDTA plasma (n=4)	90	85-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.317	2.252	2.285	2.153
500	1.969	1.923	1.946	1.814
250	1.643	1.510	1.577	1.445
125	1.211	1.191	1.201	1.069
62.5	0.832	0.784	0.808	0.676
31.2	0.554	0.537	0.546	0.414
15.6	0.325	0.305	0.315	0.183
0	0.136	0.128	0.132

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Lactobacillus paracasei L9 affects disease progression in experimental autoimmune neuritis by regulating intestinal flora structure and arginine metabolism Y Meng,Journal of neuroinflammation,2023

功能：

Receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function.; Inhibits IL-33 signaling.
基因功能参考文献：
1. administration of sST2 significantly attenuated the severity of severe acute pancreatitis and associated cardiac damage, and the cardioprotective effect is associated with its anti-inflammatory action PMID: 29653119
2. Study demonstrates that IL-33 and ST2 are highly expressed by various central nervous system (CNS) resident cells, and there was a change of the expression levels and patterns of both molecules in the CNS lesions of multiple sclerosis (MS)patients compared with healthy brain tissues. Furthermore, IL-33/ST2 signaling pathway may have its unique important function in the myelination process in the CNS compartment. PMID: 27455844
3. A strong relationship of IL-33/ST2 with natriuretic peptides and classical inflammatory mediators was observed in cardiac tissue. Expression of sST2 in cardiac, adipose and liver tissue decreased in obese rats compared with controls, suggesting an involvement for IL-33/ST2 system in molecular mechanisms of obesity. PMID: 28274612
4. these results demonstrated that ST2L translocation from the cytosol to the cell membranes of lung tissue and the down-expression of sST2 in both fractions can function as new biomarkers of (ventilator-induced lung injury. PMID: 25815839
5. This is the first study demonstrating a deleterious role for sST2 in the vascular remodeling associated with obesity. PMID: 24265755
6. Interleukin-33 prevents apoptosis and improves survival after experimental myocardial infarction through ST2 signaling. PMID: 19919994
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亚细胞定位：

Cell membrane; Single-pass type I membrane protein.; [Isoform B]: Secreted.
蛋白家族：

Interleukin-1 receptor family
组织特异性：

Isoform A is detected in spleen, lung, bone marrow and lymh node. Isoform B is predominant in fibroblasts.
数据库链接：

KEGG: rno:25556

STRING: 10116.ENSRNOP00000020144

UniGene: Rn.10072

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.