Rat Glypican-3(GPC3) ELISA kit

Rat Glypican-3(GPC3) ELISA kit

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中文名称：

大鼠磷脂酰肌醇蛋白聚糖3(GPC3)酶联免疫试剂盒
货号：

CSB-EL009705RA
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO大鼠磷脂酰肌醇蛋白聚糖3（GPC3）定量检测试剂盒，采用双抗体夹心ELISA技术，专为科研实验设计。GPC3是一种细胞膜表面的硫酸乙酰肝素蛋白聚糖，参与调控细胞增殖和分化过程，在肝脏发育及病理状态（如肝癌发生）中具有重要研究价值。试剂盒可检测血清、血浆及组织匀浆样本，线性范围为15.6-1000 pg/mL，通过预包被高特异性抗体实现目标蛋白的高效捕获，配合HRP标记检测抗体完成信号放大，实验全程约需4.5小时。该试剂盒适用于肝病模型研究、肿瘤微环境分析及靶向治疗机制探索等科研场景，例如可通过监测药物干预后GPC3表达水平变化评估治疗效果，或结合组织样本分析其在不同病理阶段的生物学功能。试剂盒提供标准品、预混试剂及优化实验方案，支持96孔板规格，重复性良好，为研究者提供便捷可靠的定量分析工具。
别名：

Gpc3 ELISA Kit; Glypican-3 ELISA Kit; Intestinal protein OCI-5) [Cleaved into: Glypican-3 alpha subunit; Glypican-3 beta subunit] ELISA Kit
缩写：

GPC3
Uniprot No.：

P13265
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat GPC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	85-99
1:2	Average %	107
	Range %	97-110
1:4	Average %	89
	Range %	83-93
1:8	Average %	99
	Range %	91-104

回收率：

The recovery of rat GPC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	84-99
EDTA plasma (n=4)	100	94-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.506	2.415	2.461	2.280
500	2.091	1.974	2.033	1.852
250	1.353	1.332	1.343	1.162
125	0.813	0.798	0.806	0.625
62.5	0.484	0.456	0.470	0.289
31.2	0.408	0.392	0.400	0.219
15.6	0.274	0.267	0.271	0.090
0	0.183	0.179	0.181

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Cell surface proteoglycan that bears heparan sulfate. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway. Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression. Inhibits the dipeptidyl peptidase activity of DPP4. Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4. Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis. Required for coronary vascular development. Plays a role in regulating cell movements during gastrulation.
基因功能参考文献：
1. Abnormal oncofetal antigen GPC-3 and GPC-3 mRNA expression in hepatocarcinogenesis may be promising molecular markers for early diagnosis of hepatocellular carcinoma. PMID: 21669573
2. Gpc3 is a marker of hepatic progenitor cells and of early liver lesions. PMID: 17117158
3. GPC3 may be a negative regulator of liver regeneration and hepatocyte proliferation, and that this regulation may involve CD81. PMID: 19574424
亚细胞定位：

Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side.
蛋白家族：

Glypican family
数据库链接：

KEGG: rno:25236

STRING: 10116.ENSRNOP00000043344

UniGene: Rn.9717

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.