Rat Fibronectin,FN ELISA Kit

Rat Fibronectin,FN ELISA Kit

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中文名称：

大鼠纤连蛋白(FN)酶联免疫试剂盒
货号：

CSB-E04553r
规格：

96T/48T
价格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO专为大鼠样本设计的纤连蛋白（Fibronectin, FN）定量检测试剂盒，采用双抗体夹心法原理，可精准检测血清、血浆、细胞培养上清及组织匀浆中的FN含量，检测灵敏度达0.78 ng/mL，线性范围为0.78-50 ng/mL。FN是一种广泛分布于细胞外基质的高分子糖蛋白，参与细胞粘附、迁移及组织修复等关键生物学过程，其表达水平变化常与炎症反应、组织损伤修复和肿瘤微环境调控等研究相关。本试剂盒通过预包被特异性捕获抗体与酶标记检测抗体的协同作用，可高效捕获样本中的FN分子并进行比色定量分析，适用于体外培养细胞分泌功能评估、组织工程模型基质成分分析，以及心血管疾病、纤维化疾病等动物模型中细胞外基质动态变化的科研探索。实验操作流程标准化，兼容常规酶标仪检测，为研究者提供稳定可靠的FN定量分析工具，助力于细胞行为机制、病理生理过程及相关药物筛选等基础研究领域的数据获取。
别名：

Fn1Fibronectin ELISA kit; FN) [Cleaved into: Anastellin] ELISA kit
缩写：

FN1
Uniprot No.：

P04937
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

0.78 ng/mL-50 ng/mL
灵敏度：

0.195 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat FN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	96
	Range %	85-100
1:2000	Average %	87
	Range %	82-93
1:4000	Average %	98
	Range %	95-100
1:8000	Average %	92
	Range %	88-94

回收率：

The recovery of rat FN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-98
EDTA plasma (n=4)	99	89-109

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
50	2.370	2.405	2.388	2.245
25	2.015	2.063	2.039	1.896
12.5	1.613	1.627	1.620	1.477
6.25	1.115	1.136	1.126	0.983
3.12	0.685	0.679	0.682	0.539
1.56	0.432	0.467	0.450	0.307
0.78	0.278	0.291	0.285	0.142
0	0.142	0.144	0.143

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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功能：

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.; Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
亚细胞定位：

Secreted, extracellular space, extracellular matrix.
组织特异性：

Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix.
数据库链接：

STRING: 10116.ENSRNOP00000019772

UniGene: Rn.1604

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.