Rat Fatty-acid amide hydrolase 1(FAAH) ELISA kit

产品详情

产品描述：

大鼠脂肪酸酰胺水解酶1(FAAH)酶联免疫试剂盒（CSB-EL007938RA）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FAAH含量。FAAH即脂肪酸酰胺水解酶，是一种能降解内源性大麻素的酶。其背景在于它在体内的内源性大麻素系统中发挥关键作用。研究机制主要聚焦在通过抑制FAAH活性，提高内源性大麻素水平，以探索在镇痛、抗炎、治疗精神疾病等方面的应用。试剂盒检测范围为0.156 ng/mL-10 ng/mL，本试剂盒适用于科研领域中对FAAH表达动态的定量分析，例如探究神经退行性疾病模型、代谢综合征或炎症相关动物实验中FAAH的生物学作用，也可用于评估药物或化合物对FAAH活性的调控效果。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Faah; Faah1; Fatty-acid amide hydrolase 1; Anandamide amidase; Anandamide amidohydrolase 1; Fatty acid ester hydrolase; Oleamide hydrolase 1
缩写：

FAAH
Uniprot No.：

P97612
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat FAAH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-91
1:2	Average %	94
	Range %	89-98
1:4	Average %	107
	Range %	103-110
1:8	Average %	89
	Range %	85-94

回收率：

The recovery of rat FAAH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	103	98-108

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Catalyzes the hydrolysis of endogenous amidated lipids like the sleep-inducing lipid oleamide ((9Z)-octadecenamide), the endocannabinoid anandamide (N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine), as well as other fatty amides, to their corresponding fatty acids, thereby regulating the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates. It can also catalyze the hydrolysis of the endocannabinoid 2-arachidonoylglycerol (2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol). FAAH cooperates with PM20D1 in the hydrolysis of amino acid-conjugated fatty acids such as N-fatty acyl glycine and N-fatty acyl-L-serine, thereby acting as a physiological regulator of specific subsets of intracellular, but not of extracellular, N-fatty acyl amino acids.
亚细胞定位：

Endoplasmic reticulum membrane; Single-pass membrane protein. Golgi apparatus membrane; Single-pass membrane protein. Note=Seems to be associated with the endoplasmic reticulum and/or Golgi apparatus.
蛋白家族：

Amidase family
组织特异性：

Found in neuronal cells throughout the CNS. Expressed in liver and brain, and to a lesser extent in spleen, lung, kidney and testes.
数据库链接：

KEGG: rno:100911581

STRING: 10116.ENSRNOP00000015961

UniGene: Rn.89119