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中文名称:大鼠肾上腺素(EPI)酶联免疫试剂盒
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货号:CSB-E08678r
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规格:96T/48T
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价格:¥3900/¥2500
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其他:
产品详情
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产品描述:CUSABIO大鼠肾上腺素(EPI)酶联免疫检测试剂盒(货号:CSB-E08678r),适用于定量分析血清、血浆及组织匀浆样本中EPI含量,检测灵敏度为1 pg/mL,线性范围为1-200 pg/mL。肾上腺素作为关键的儿茶酚胺类激素和神经递质,广泛参与机体应激反应、心血管功能调节及能量代谢等生理过程。本试剂盒基于竞争法原理设计,样本中的EPI与预先包被的标准品竞争结合特异性抗体,通过显色反应强度反比推算目标物浓度,操作流程包含样本预处理、孵育、洗涤及比色测定等步骤,试剂盒提供预包被微孔板、系列浓度标准品、检测抗体、显色底物及终止液等完整组分,3.5小时内即可完成检测。该产品可满足神经生物学、药理学及内分泌学等领域的基础研究需求,例如探索应激状态下激素释放机制、心血管疾病模型中交感神经活性评估,或代谢综合征相关动物实验的EPI动态监测,为科研人员提供稳定可靠的定量分析工具。
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缩写:EPI
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种属:Rattus norvegicus (Rat)
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样本类型:serum, plasma, tissue homogenates
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检测范围:1 pg/mL-200 pg/mL
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灵敏度:0.5 pg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Neuroscience
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测定原理:quantitative
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测定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat EPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 98 Range % 90-103 1:2 Average % 90 Range % 85-96 1:4 Average % 94 Range % 89-99 1:8 Average % 85 Range % 80-90 -
回收率:
The recovery of rat EPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 89-98 EDTA plasma (n=4) 91 85-96 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average 200 0.203 0.202 0.203 50 0.378 0.351 0.365 16 0.581 0.537 0.559 4 0.945 1.012 0.979 1 1.416 1.493 1.455 0 2.241 2.367 2.304 -
数据处理:
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货期:3-5 working days
引用文献
- CP-25 inhibits the hyperactivation of rheumatic synoviocytes by suppressing the switch in Gαs-Gαi coupling to the β2-adrenergic receptor M Ge,Cell communication and signaling,2023
- Paroxetine Attenuates Cardiac Hypertrophy Via Blocking GRK2 and ADRB1 Interaction in Hypertension X Sun,Journal of the American Heart Associatio,2021
- Swim therapy-induced tissue specific metabolic responses in male rats A Moustafa,Life Sciences,2020
- Effects of varying intensities of heat stress on neuropeptide Y and proopiomelanocortin mRNA expression in rats N Zhao,Biomedical Reports,2020
- Effects of β‐adrenoceptor activation on hemodynamics in hypoxic stress rats Q Ji,Exp. Physiol,2020
- Effect of Solanum macrocarpon Linn leaf aqueous extract on the brain of an alloxan-induced rat model of diabetes MA Okesola,J Int Med Res,2020
- Low-intensity focused ultrasound stimulation treatment decreases blood pressure in spontaneously hypertensive rats D Li,IEEE Transactions on Biomedical Engineering,2020
- Acute restraint stress alters food-foraging behavior in rats: Taking the easier Way while suffered Tu BX,Brain Research Bulletin,2019
- Antidepressant Effect of a Polyherbal Syrup Based on Iranian Traditional Medicine Sara Zakerin, et al,Research journal of pharmacognos,2019
- Transplantation of bone marrow mesenchymal stem cells reduces GluR1 expression in the hippocampus and amygdala and affects secretion of stress-related hormones in rats with spinal cord injuries: a potential anti-chronic stress mechanism Jia-Jia Sun.et al,INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE,2018
- Exercise Training Induced Cardioprotection with Moderate Hyperglycemia versus Sedentary Intensive Glycemic Control in Type 1 Diabetic Rats Matthew W. McDonald.et al,Journal of Diabetes Research,2018
- Acute bout of exercise induced prolonged muscle glucose transporter-4 translocation and delayed counter-regulatory hormone response in type 1 diabetes Koji Sato.et al,PLoS One,2017
- Neuropeptide S increases motor activity and thermogenesis in the rat through sympathetic activation Ensho T.et al,Neuropeptides.,2017
- The glucoregulatory response to high-intensity aerobic exercise following training in rats with insulin-treated type 1 diabetes mellitus. McDonald MW.et al,Appl Physiol Nutr Metab.,2016
- Ozone Inhalation Provokes Glucocorticoid-Dependent and -Independent Effects on Inflammatory and Metabolic Pathways. Thomson EM.et al,Toxicol Sci.,2016
- Estrogen resisted stress-induced cardiomyopathy through increasing the activity of β Cao X.et al,Int J Cardiol.,2015
- The glucoregulatory response to high intensity aerobic exercise following training in rats with insulin-treated Type 1 Diabetes Mellitus McDonald M W.et al,Applied Physiology, Nutrition, and Metabolism,2016
- The glucoregulatory response to high intensity aerobic exercise following training in rats with insulin-treated Type 1 Diabetes Mellitus Matthew W McDonald. et al, Applied Physiology, Nutrition, and Metabolism,2016
- Influence of Endurance Exercise Overloading Patterns Levels Ventricular Catecholamines After a Bout of Lactate Threshold Test in Male Wistar Rat Ahmad Azad . et al,Asian Journal of Sports Medicine,2015
- Glycyrrhizic acid prevents high calorie diet-induced metabolic aberrations despite the suppression of peroxisome proliferator-activated receptor 纬 expression Hong Sheng Cheng.et al,Nutrition,2016
- Estrogen resisted stress-induced cardiomyopathy through increasing the activity of ß 2 AR-Gas signal pathway in female rats Xiancun Cao et al,International Journal of Cardiology,2015
- Ozone Induces Glucose Intolerance and Systemic Metabolic Effects in Young and Aged Brown Norway Rats Bass V et al,Toxicol Appl Pharmacol,2013
- Stress and its Effects on Glucose Metabolism and 11ß-HSD Activities in Rats Fed on a Combination of High-Fat and High-Sucrose Diet with Glycyrrhizic Acid Fernando HA et al,J Diabetes Res,2013
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靶点详情
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最新研究进展:EPI是一种激素,主要由肾上腺分泌,具有增强心脏功能、提高血糖水平等生理作用。最新研究表明,EPI与多种疾病有关,如心血管疾病、代谢综合征、糖尿病等。一些研究还表明,EPI还可能参与肿瘤的发生和发展。未来的研究将继续探讨EPI在疾病中的作用机制,以及开发针对EPI的治疗方法。
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