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Rat Epidermal growth factor,EGF ELISA Kit

  • 中文名称:
    大鼠表皮生长因子(EGF)酶联免疫试剂盒
  • 货号:
    CSB-E08029r
  • 规格:
    96T/48T
  • 价格:
    ¥3200/¥2500
  • 其他:

产品详情

  • 产品描述:
    CUSABIO专为大鼠样本设计的表皮生长因子(EGF)酶联免疫检测试剂盒(货号:CSB-E08029r),采用双抗体夹心酶联免疫吸附法进行定量检测,可精准测定血清、血浆及组织匀浆中的EGF浓度。EGF作为重要的细胞生长调节因子,广泛参与表皮细胞增殖、分化及组织修复等生物学过程,其表达水平与皮肤再生、黏膜修复及部分病理过程密切相关。本试剂盒检测灵敏度高,线性范围为3.12-200 pg/mL,最低检测限达3.12 pg/mL,可满足科研场景中微量样本的检测需求,适用于大鼠疾病模型研究、药物干预效果评估或基础科研中EGF动态变化的定量分析。通过优化抗体配对及反应体系,试剂盒可在4小时内完成检测流程,配套提供标准品、预包被酶标板及检测所需全套组分,支持科研人员高效开展与皮肤损伤修复、肿瘤微环境或炎症调控相关的分子机制研究,为探索EGF在大鼠生理及病理过程中的作用提供可靠工具。
  • 别名:
    Egf ELISA Kit; Pro-epidermal growth factor ELISA Kit; EGF) [Cleaved into: Epidermal growth factor] ELISA Kit
  • 缩写:
  • Uniprot No.:
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    3.12 pg/mL-200 pg/mL
  • 灵敏度:
    0.78 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Others
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of rat EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:100

    Average %

    91

    Range %

    86-98

    1:200

    Average %

    96

    Range %

    92-104

    1:400

    Average %

    85

    Range %

    81-88

    1:800

    Average %

    88

    Range %

    83-94

  • 回收率:

    The recovery of rat EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    90

    87-94

    EDTA plasma (n=4)

    92

    88-96

  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    200

    2.503

    2.478

    2.491

    2.347

    100

    2.156

    2.168

    2.162

    2.018

    50

    1.564

    1.560

    1.562

    1.418

    25

    1.024

    1.001

    1.013

    0.869

    12.5

    0.586

    0.549

    0.568

    0.424

    6.25

    0.364

    0.345

    0.355

    0.211

    3.12

    0.258

    0.264

    0.261

    0.117

    0

    0.149

    0.138

    0.144

     

  • 数据处理:
  • 货期:
    3-5 working days

产品评价

靶点详情

  • 功能:
    EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6.
  • 基因功能参考文献:
    1. The expression of EGF was elevated on the first day after tympanic membrane (TM)injury, not reaching statistical significance, and then returned to the level observed in the control TM. PMID: 25920966
    2. A decrease in EGF levels may contribute to the formation of visceral hypersensitivity through downregulation of SERT-mediated 5-HT uptake into enterocytes. PMID: 25309082
    3. The role of EGF on apoptosis, cell proliferation, oxidative stress and antioxidant system in intestinal injury induced by ischemia/reperfusion in rats. PMID: 23932386
    4. results indentify EGF signalling as a robust vasculogenic inductive pathway for ATMCs, leading to their transdifferentiation into functional VSMC-like cells. PMID: 24967966
    5. Epidermal growth factor and erythropoietin infusion accelerate functional recovery in combination with rehabilitation. PMID: 24763928
    6. these findings indicate that cortical EGF levels decrease with a high-salt diet in salt-sensitive rats, promoting ENaC-mediated Na(+) reabsorption in the collecting duct and the development of hypertension. PMID: 23599382
    7. Epidermal growth factor modulates voltage sensitivity of slow sodium channels. PMID: 23821047
    8. Changes in colonic permeability during low-sodium adaptation are mediated by the EGF secreted by myofibroblasts in response to raised aldosterone. PMID: 23467299
    9. findings indicate NGF is an important regulator for the synthesis and secretion of EGF and insulin from beta cells. NGF withdrawal causes apoptosis by decreasing EGF, NGF and insulin secretion from beta cells of hyperglycemic rats. PMID: 22926925
    10. nucleobindin-2 regulates EGF-stimulated MAPK kinase/Erk signaling, cell proliferation, and adipocyte differentiation PMID: 22514047
    11. There were no differences in the Egf expression between the rat strains at rest and under emotional stress in both the cortical and medullary kidney zones. PMID: 22645946
    12. NGF, GDNF and EGF stimulation of DRG-neurons shows differential Erk1/2 activation responses and a corresponding differential behavioral phenotype; EGF did not induce mechanical hyperalgesia, but blocked PGE2-induced sensitization PMID: 21187008
    13. EGF plays multiple roles in rat tympanic membrane (TM) wound healing. PMID: 20967565
    14. EGF also activates focal adhesion kinase in the cytoplasm to rephosphorylate Grb7, releasing KOR mRNA for active translation. PMID: 20142421
    15. epidermal growth factor (EGF) can be converted from a non-neuritogenic into a neuritogenic factor through moderate activation of endogenous SRC by receptor-protein-tyrosine phosphatase alpha (a physiological SRC activator) PMID: 11867627
    16. stimulation of recruits epidermal growth factor receptor and extracellular signal-regulated kinases to down-regulate tropoelastin mRNA in lung fibroblasts PMID: 11889128
    17. isoproterenol treatment leads to a hyperstimulatory state of granular convoluted tubule cells, which then causes depletion of cellular stores of mature EGF, and most likely due to a shortened posttranslational transit, incomplete peptide processing PMID: 12047917
    18. lymph node stromal cell lines expressed mRNA transcripts for IGF-I and EGF as major mitogenic factors which may contribute actively to the process of cancer cell dissemination PMID: 12115579
    19. EGF is internalized into clathrin-coated vesicles by NLRR-3, resulting in Ras-MAPK signaling PMID: 12297494
    20. EGF plays a role in T3-induced cerebellar development in astrocytes PMID: 12356760
    21. EGF- and Src-mediated transactivation pathway for IGF-IR activation in hepatocytes, and indicate a role for the IGF-IR in hepatocyte intracellular signaling. PMID: 12447877
    22. both epidermal growth factor and thyrotropin releasing hormone stimulated prolactin gene transcription appear to require protein kinase C delta, whereas protein kinase C eta may also be able to transmit the epidermal growth factor response PMID: 12456804
    23. Mucosal permeability regulates receptor binding of luminal EGF in the adult rat small intestine. PMID: 12579338
    24. IGF1 and EGF synergistically suppress apoptosis in salivary acinar cells PMID: 12700634
    25. role in divergence in signaling pathways involved in promotion of cell viability PMID: 12834262
    26. EGF and angiotensin II activation of phospholipase Cgamma through src is mediated by GIT1 PMID: 14523024
    27. EGF-mediated regulation of PEPT2 expression in a kidney cell line PMID: 14559717
    28. epidermal growth factor-induced signaling sensitization by bradykinin is mediated by c-Src PMID: 14630916
    29. Mechanisms by which EGF reduced angiotensin II surface receptor density and stimulated actions in vascular smooth muscle cells were studied. PMID: 15254973
    30. Epidermal growth factor triggers caspase-independent pituitary cell death PMID: 15331766
    31. These results suggest that increases in transepithelial resistance accompanying AEC transdifferentiation and/or EGF exposure are mediated, at least in part, by changes in the pattern of expression of specific claudin isoforms. PMID: 15361518
    32. This entire ectodomain is further processed into a 70-kDa precursor and finally into the mature 5.9 kDa urinary EGF PMID: 15380929
    33. Linear signal transduction pathway involving PI3-kinase-dependent MAPK regulates EGF-induced DNA synthesis in mesangial cells by regulating c-fos and p27(Kip1) expression. PMID: 15701816
    34. The Epidermal Growth Factor induces transient and sustained ERK activation. PMID: 15793571
    35. The epidermal growth factor acts to cell differentiate and proliferate of PC12 cells. PMID: 15803132
    36. Neo-natal rats with intestinal injury have significantly lower levels of ileum EGF. PMID: 15968738
    37. ERK1/2 mediates EGF-induced rapid Rb phosphorylation at Ser780 and Ser795 PMID: 16126730
    38. EGF-based signaling cascades phosphorylate Cav1 and have roles in caveolae assembly PMID: 16332692
    39. Alpha(1)-adrenergic agonists activate the alpha(1D)-AR adrenergic receptors to stimulate protein secretion and the ectodomain shedding of epidermal growth factor (EGF) to transactivate the EGF receptor. PMID: 16760267
    40. It is concluded that EGF regulates pax2 & GAPDH abundance & proteolysis in renal tubular cells through a PI3K/Akt-sensitive pathway that does not involve mTOR PMID: 16885413
    41. betaFGF, EGF and TGFbeta up-regulated the VEGF/FLK-1 receptor system in rat pituitary GH3 cells PMID: 16944045
    42. proliferative capability of hepatocytes was impaired by continuous liver damage due, in part, to the decrease of a hepatocyte mitogen EGF, and that increased intrahepatic TGF-beta1 activated hepatic stellate cells PMID: 17203207
    43. Wound healing was suppressed by one-half upon epidermal growth factor titration with EGF-antibody. PMID: 17631610
    44. SO(2) increasedthe expressions of EGF, EGFR, and COX-2 on the transcription and translation levels in the lungs and tracheas from asthmatic rats. PMID: 17962899
    45. Cat.G-induced cardiomyocyte apoptosis involves an increase in EGFR-dependent activation of SHP2 that promotes focal adhesion kinase dephosphorylation and subsequent cardiomyocyte anoikis. PMID: 17975113
    46. specific signaling functions and induction of genes differentially required for enhanced effect of EGF- and TGF-beta1-induced oncogenic properties PMID: 17982486
    47. Enhancement of PPARgamma activity by curcumin interrupted PDGF and EGF signaling in activated hepatic stellate cells by reducing the phosphorylation levels of PDGF-betaR and EGFR, and by suppressing the receptor gene expression. PMID: 18332871
    48. Decreased food intake did not affect the expression of EGF, PTHLH, or LEP mRNA in mammary tissue. PMID: 18401831
    49. EGFR inhibition enhances apoptosis induced by TGF-beta in FaO rat hepatoma cells through an increased oxidative stress coincident with a change in the expression pattern of NOX enzymes. PMID: 18848961
    50. demonstrated a unique role for EGF as a mediator of dopamine-induced precursor cell proliferation in the subventricular zone PMID: 19433789

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  • 亚细胞定位:
    Membrane; Single-pass type I membrane protein.
  • 数据库链接:

    KEGG: rno:25313

    STRING: 10116.ENSRNOP00000040920

    UniGene: Rn.6075