Rat Dehydroepiandrosterone sulfate,DHEA-S ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

大鼠脱氢表雄酮硫酸酯(DHEA-S)酶联免疫试剂盒（CSB-E05106r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的DHEA-S含量。DHEA-S即硫酸脱氢表雄酮，是肾上腺皮质分泌的一种激素前体。它在人体生理活动中起重要作用，如影响代谢、免疫等。研究机制上，其可转化为雄激素和雌激素，参与调节多种生理功能，对它的研究有助于深入了解人体内分泌及相关疾病机制。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，本产品适用于动物模型中的激素水平监测、代谢性疾病机制研究、应激生理学分析等科研场景，例如通过检测大鼠血清/血浆DHEA - S浓度，探究肾上腺皮质功能状态或激素相关疾病的分子调控网络，为内分泌领域的机制研究提供高效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

DHEA-S
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat DHEA-S in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	87
	Range %	81-92
1:200	Average %	96
	Range %	90-100
1:400	Average %	95
	Range %	91-104
1:800	Average %	92
	Range %	84-98

回收率：

The recovery of rat DHEA-S spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-97
EDTA plasma (n=4)	97	91-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.