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  4. Rat Cytochrome c(CYCS) ELISA kit

Rat Cytochrome c(CYCS) ELISA kit

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  • 中文名称：
    大鼠细胞色素C(CYCS)酶联免疫试剂盒
  • 货号：
    CSB-EL006328RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO大鼠细胞色素C（CYCS）酶联免疫检测试剂盒（货号：CSB-EL006328RA），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中的CYCS含量，检测范围为78-5000 pg/mL。细胞色素C是线粒体电子传递链的关键成分，同时参与调控细胞凋亡过程，其表达水平变化与能量代谢异常、氧化应激及程序性细胞死亡等生物学机制密切相关。本试剂盒通过高特异性抗体对实现精准检测，适用于大鼠模型中CYCS相关的基础研究，例如线粒体功能评估、凋亡信号通路探索、药物诱导的细胞损伤研究或心血管疾病模型的分子机制分析。实验操作兼容常规实验室条件，支持多样本类型检测，为科研人员提供稳定可靠的工具，助力揭示CYCS在生理及病理状态下的动态变化规律。
  • 别名：
    CycsCytochrome c ELISA Kit; somatic ELISA Kit
  • 缩写：
    CYCS
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    78 pg/mL-5000 pg/mL
  • 灵敏度：
    19.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CYCS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %90
    Range %87-94
    1:2Average %89
    Range %85-96
    1:4Average %92
    Range %90-95
    1:8Average %91
    Range %87-96
  • 回收率：
    The recovery of rat CYCS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-96
    EDTA plasma (n=4)9084-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    50002.581 2.458 2.520 2.427
    25002.234 2.186 2.210 2.117
    12501.638 1.621 1.630 1.537
    6251.103 1.097 1.100 1.007
    3120.692 0.672 0.682 0.589
    1560.393 0.386 0.390 0.297
    780.236 0.223 0.230 0.137
    00.094 0.092 0.093 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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Cycs Proteins

靶点详情

  • 功能：
    Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.; Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
  • 亚细胞定位：
    Mitochondrion intermembrane space. Note=Loosely associated with the inner membrane.
  • 蛋白家族：
    Cytochrome c family
  • 组织特异性：
    Found in embryos and in adult liver and heart.
  • 数据库链接：

    KEGG: rno:100363502

    STRING: 10116.ENSRNOP00000041521

    UniGene: Rn.161841

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |