Rat Cytochrome P450 19A1(CYP19A1) ELISA kit

Rat Cytochrome P450 19A1(CYP19A1) ELISA kit

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中文名称：

大鼠细胞色素P450 19A1(CYP19A1)酶联免疫试剂盒
货号：

CSB-EL006394RA
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

大鼠细胞色素P450 19A1(CYP19A1)酶联免疫试剂盒（CSB-EL006394RA）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CYP19A1含量。CYP19A1是一种编码芳香化酶的基因，位于细胞色素P450超家族，主要在肝脏和脂肪组织中表达。该酶在雌激素的生物合成中起关键作用，其活性异常与多种癌症的发生发展密切相关。研究显示，CYP19A1通过调控脂代谢和免疫应答在肿瘤中发挥作用，具有遗传多样性，其突变可能影响雌激素水平和细胞凋亡。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，适用于体外分析大鼠内分泌调控机制、卵巢生理病理变化、环境污染物对激素代谢的影响等研究，为生殖生物学、毒理学及激素相关疾病模型的建立提供定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Cyp19a1 ELISA Kit; Arom ELISA Kit; Cyp19Aromatase ELISA Kit; EC 1.14.14.14 ELISA Kit; CYPXIX ELISA Kit; Cytochrome P-450AROM ELISA Kit; Cytochrome P450 19A1 ELISA Kit; Estrogen synthase ELISA Kit
缩写：

CYP19A1
Uniprot No.：

P22443
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

18.75 pg/mL-1200 pg/mL
灵敏度：

4.68 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CYP19A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	101
	Range %	95-107
1:2	Average %	91
	Range %	86-97
1:4	Average %	88
	Range %	84-92
1:8	Average %	95
	Range %	90-100

回收率：

The recovery of rat CYP19A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	80-90
EDTA plasma (n=4)	96	91-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.452	2.272	2.362	2.237
600	1.705	1.832	1.769	1.644
300	1.286	1.316	1.301	1.176
150	0.829	0.880	0.855	0.730
75	0.504	0.469	0.487	0.362
37.5	0.301	0.285	0.293	0.168
18.75	0.203	0.211	0.207	0.082
0	0.127	0.122	0.125

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Prochloraz Decreases Aromatase Activity and Serum Concentrations of E2, Testosterone, T3, and T4 in Pubertal Developmental Rats SY Lu,Taiwan Pesticide Science,2022
Tebuconazole Disrupted Aromatase Activity and Reduced 17β-Estradiol Concentration in Pubertal Developmental Rats SY Lu,Taiwan Pesticide Science,2020
PROTECTIVE EFFECTS OF CACTUS AND/OR PAPAYA JUICES AGAINST HEPATIC AND TESTICULAR TOXICITY INDUCED BY CHLORPYRIFOS IN ALBINO RATS Mogahed H E, et al,World Journal of Pharmaceutical Research,2019
Deltamethrin Alters Thyroid Hormones and Delays Pubertal Development in Male and Female Rats Pinpin Lin, et al,Comparative Endocrinology of Animals,2018

功能：

Catalyzes the formation of aromatic C18 estrogens from C19 androgens.
亚细胞定位：

Membrane; Peripheral membrane protein.
蛋白家族：

Cytochrome P450 family
数据库链接：

STRING: 10116.ENSRNOP00000000212

UniGene: Rn.21402

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.