Rat Complement fragment 5,C5 ELISA Kit

Rat Complement fragment 5,C5 ELISA Kit

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中文名称：

大鼠补体片断5(C5)酶联免疫试剂盒
货号：

CSB-E14363r
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO专用于科研领域的大鼠补体片段5（C5）酶联免疫检测试剂盒（货号：CSB-E14363r），基于双抗体夹心法原理实现样本中C5蛋白的定量分析。补体C5是补体系统激活途径中的关键成分，其裂解产物C5a和C5b在炎症反应、细胞趋化和膜攻击复合物形成中发挥重要作用，对研究免疫调控、感染性疾病或炎症相关机制具有重要价值。本试剂盒检测灵敏度为9.38 ng/mL，线性检测范围覆盖9.38-600 ng/mL，适用于血清、血浆及组织匀浆样本的检测，实验前需对高浓度样本进行适当稀释或离心处理。检测流程通过预包被于微孔板的特异性抗体捕获样本中的C5蛋白，结合标记抗体后显色，最终通过标准曲线计算目标浓度。该试剂盒可应用于大鼠模型中的补体系统功能研究，例如评估炎症性疾病动物模型的病理进程、药物干预对补体激活的影响，或探究组织损伤修复过程中C5的动态变化，为免疫学、药理学及基础医学研究提供可靠工具。
别名：

C5Complement C5 [Cleaved into: C5a anaphylatoxin] ELISA kit; Fragment ELISA kit
缩写：

C5
Uniprot No.：

P08650
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

9.38 ng/mL-600 ng/mL
灵敏度：

2.34 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat C5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:200	Average %	104
	Range %	97-111
1:400	Average %	101
	Range %	94-108
1:800	Average %	98
	Range %	93-103
1:1600	Average %	91
	Range %	84-97

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

600

2.218

2.134

2.176

2.070

300

1.864

1.767

1.815

1.710

150

1.346

1.298

1.322

1.216

0.937

0.929

0.933

0.827

37.5

0.601

0.611

0.606

0.500

18.75

0.436

0.453

0.445

0.339

9.38

0.302

0.294

0.298

0.192

0.106

0.105

0.106

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

iTRAQ-based proteomic analysis to identify the molecular mechanism of Zhibai Dihuang Granule in the Yin-deficiency-heat syndrome rats Liu CM.et al,Chin Med,2018

功能：

Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.; Derived from proteolytic degradation of complement C5, C5a anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
亚细胞定位：

Secreted.
数据库链接：

STRING: 10116.ENSRNOP00000025534

UniGene: Rn.21259

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.