Rat Coagulation factor VII(F7) ELISA kit

中文名称：

大鼠凝血因子VII(F7)酶联免疫试剂盒
货号：

CSB-EL007930RA
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO大鼠凝血因子VII（F7）酶联免疫检测试剂盒（货号：CSB-EL007930RA），基于双抗体夹心法原理实现精准定量分析，适用于血清、血浆及组织匀浆样本中F7蛋白的检测。凝血因子VII是外源性凝血途径的关键启动因子，由肝脏合成并参与止血与血栓形成调控，其水平变化与凝血功能异常、肝损伤及心血管疾病模型研究密切相关。试剂盒检测灵敏度达0.195 ng/mL，线性范围为0.78-50 ng/mL，采用预包被96孔板形式，仅需3小时即可完成检测流程，操作便捷高效。试剂盒包含标准品、检测抗体及显色底物等全套组分，支持科研领域对凝血机制、药物作用靶点筛选、肝脏功能评估等研究需求。试剂在2-8℃条件下可稳定保存6个月，开盖后各组分需按说明书要求分装冻存以避免反复冻融。本产品严格验证批次间一致性，为凝血相关基础研究提供可靠工具。
别名：

F7Coagulation factor VII ELISA Kit; EC 3.4.21.21 ELISA Kit; Serum prothrombin conversion accelerator) [Cleaved into: Factor VII light chain; Factor VII heavy chain] ELISA Kit
缩写：

F7
Uniprot No.：

Q8K3U6
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.78 ng/mL-50 ng/mL
灵敏度：

0.195 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Blood Coagulation
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat F7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	91
	Range %	86-98
1:400	Average %	97
	Range %	92-106
1:800	Average %	93
	Range %	88-99
1:1600	Average %	89
	Range %	83-95

回收率：

The recovery of rat F7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	109	103-115

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
50	2.468	2.652	2.560	2.424
25	2.115	2.076	2.096	1.960
12.5	1.503	1.504	1.504	1.368
6.25	0.845	0.805	0.825	0.689
3.12	0.526	0.524	0.525	0.389
1.56	0.381	0.359	0.370	0.234
0.78	0.274	0.259	0.267	0.131
0	0.137	0.135	0.136

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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功能：

Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
基因功能参考文献：
1. The EGF(1) region of rat coagulation factor can mediate the specific binding of FVII with tissue factor. PMID: 18315926
亚细胞定位：

Secreted.
蛋白家族：

Peptidase S1 family
组织特异性：

Plasma.
数据库链接：

KEGG: rno:260320

STRING: 10116.ENSRNOP00000038466

UniGene: Rn.86416

Rat Coagulation factor VII(F7) ELISA kit

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