Rat Cluster of Differentiation 68, CD68 ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠CD68分子定量检测酶联免疫试剂盒（货号：CSB-E13297r），采用双抗体夹心法原理，可精准检测血清、血浆及组织匀浆样本中0.625-40 ng/mL浓度范围的CD68蛋白。CD68作为巨噬细胞特异性表面标志物，广泛表达于单核/巨噬细胞系的溶酶体膜，在固有免疫应答、炎症调控及抗原提呈过程中发挥关键作用。该试剂盒适用于多种科研场景，包括巨噬细胞活化状态分析、炎症性疾病动物模型研究（如关节炎或动脉粥样硬化）、组织修复过程中巨噬细胞浸润评估等实验需求。试剂盒操作便捷，可在4.5小时内完成检测流程，配套标准品与预包被板提供稳定检测体系，支持科研人员高效开展免疫细胞功能研究、病理机制探索及药物干预效果评价等实验，尤其适用于心血管、肿瘤微环境及免疫代谢相关领域的样本检测。
缩写：

CD68
Uniprot No.：

Q4FZY1
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.625 ng/mL-40 ng/mL
灵敏度：

0.156 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CD68 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	86-96
1:2	Average %	102
	Range %	98-105
1:4	Average %	91
	Range %	87-95
1:8	Average %	95
	Range %	91-98

回收率：

The recovery of rat CD68 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-92
EDTA plasma (n=4)	101	94-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.