Rat Chordin-like protein 1(CHRDL1) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

大鼠腱蛋白样蛋白1(CHRDL1)酶联免疫试剂盒（CSB-EL005378RA）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CHRDL1含量。试剂盒检测范围为0.45 ng/mL-30 ng/mL，适用于基础科研中 CHRDL1 表达调控机制研究、骨骼发育模型建立、神经再生实验中的蛋白动态分析，或病理状态下组织修复相关研究。96 孔板规格设计满足高通量筛选需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Chrdl1 ELISA Kit; Kjr ELISA Kit; Ng1Chordin-like protein 1 ELISA Kit; Kohjirin ELISA Kit; Neuralin-1 ELISA Kit; Neurogenesin-1 ELISA Kit; Ventroptin ELISA Kit
缩写：

CHRDL1
Uniprot No.：

Q76LD0
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.45 ng/mL-30 ng/mL
灵敏度：

0.11 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CHRDL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-96
1:2	Average %	96
	Range %	91-104
1:4	Average %	97
	Range %	92-105
1:8	Average %	95
	Range %	89-99

回收率：

The recovery of rat CHRDL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-108
EDTA plasma (n=4)	91	86-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Seems to antagonize the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation. May play a role in embryonic bone formation. Plays a role during anterior segment eye development.
基因功能参考文献：
1. Ng1 gene could promote the proliferation of endogenous neural stem cells and protect injured neurons following spinal cord injury. PMID: 20459000
2. Neurogenesin-1 contributes to neuronal differentiation of neural stem cells in the adult brain PMID: 14684875
亚细胞定位：

Secreted.
数据库链接：

KEGG: rno:363455

STRING: 10116.ENSRNOP00000006008

UniGene: Rn.211154