Rat Chitinase-3-like protein 1(CHI3L1) ELISA kit

Rat Chitinase-3-like protein 1(CHI3L1) ELISA kit

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中文名称：

大鼠几丁质酶3样蛋白1(CHI3L1)酶联免疫试剂盒
货号：

CSB-EL005346RA
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO试剂盒采用双抗体夹心酶联免疫法检测大鼠血清、血浆及组织匀浆样本中的几丁质酶3样蛋白1（CHI3L1），该蛋白在炎症反应、组织修复及纤维化过程中发挥重要调控作用，是研究呼吸系统疾病、肝纤维化及肿瘤微环境等病理机制的关键生物标志物。试剂盒检测范围为4.7-300 ng/mL，最低检测限为4.7 ng/mL，可精准分析不同样本中CHI3L1的浓度变化。实验操作采用预包被特异性抗体的96孔板，通过标准品梯度稀释建立定量曲线，结合HRP标记检测抗体及TMB显色系统实现靶蛋白的定量分析，适用于炎症相关疾病模型构建、纤维化进程评估及药物干预效果研究等科研领域。样本兼容性广泛，支持血清直接检测及组织匀浆离心上清液分析，为探索CHI3L1在生理病理过程中的分子机制提供可靠工具。所有组分经严格质控，包含标准品、检测抗体、预混显色液及终止液等完整配套试剂，确保实验稳定性和重复性。
别名：

Chi3l1Chitinase-3-like protein 1 ELISA kit; Cartilage glycoprotein 39 ELISA kit; CGP-39 ELISA kit; GP-39 ELISA kit
缩写：

CHI3L1
Uniprot No.：

Q9WTV1
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

4.7 ng/ml - 300 ng/ml
灵敏度：

1.17 ng/ml
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CHI3L1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	103
	Range %	99-108
1:2	Average %	98
	Range %	94-102
1:4	Average %	93
	Range %	89-97
1:8	Average %	90
	Range %	86-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

300

2.486

2.358

2.422

2.241

150

2.043

1.931

1.987

1.806

1.241

1.329

1.285

1.104

37.5

0.765

0.803

0.784

0.603

18.75

0.413

0.427

0.420

0.239

9.4

0.339

0.345

0.342

0.161

4.7

0.231

0.224

0.228

0.047

0.180

0.182

0.181

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

The Effects of Ferulic Acid Against Oxidative Stress and Inflammation in Formaldehyde-Induced Hepatotoxicity. Gerin F.et al,Inflammation.,2016
The Effects of Quercetin on Acute Lung Injury and Biomarkers of Inflammation and Oxidative Stress in the Rat Model of Sepsis Gerin F. et al,Inflammation,2015

功能：

Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.
基因功能参考文献：
1. Results showed that protein and mRNA levels of CHI3L1 decreased in normal cartilage but increased in osteoarthritic cartilage. PMID: 26978347
2. NGF-treated tissue showed increased expression of CHI3L1 which was inhibited by the presence of Ro 08-2750 in intervertebral disk cells. PMID: 25062286
3. Data show that hepatic CD14+ cells are an YKL-40(Chi3L1)mRNA and protein source in acute and chronic liver injury, with expression patterns similar to growth factors implicated in inflammation-fibrogenesis PMID: 21987626
亚细胞定位：

Secreted, extracellular space. Cytoplasm. Cytoplasm, perinuclear region. Endoplasmic reticulum.
蛋白家族：

Glycosyl hydrolase 18 family
数据库链接：

KEGG: rno:89824

STRING: 10116.ENSRNOP00000065787

UniGene: Rn.68940

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.