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中文名称:大鼠胱天蛋白酶9(Casp-9)酶联免疫试剂盒
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货号:CSB-E08863r
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规格:96T/48T
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价格:¥3900/¥2500
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其他:
产品详情
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产品描述:CUSABIO特异性检测大鼠胱天蛋白酶9(Caspase-9)的定量ELISA试剂盒,采用双抗体夹心法原理,适用于血清、血浆及组织匀浆样本的检测。Caspase-9是半胱氨酸蛋白酶家族成员,在细胞凋亡的线粒体途径中作为关键启动酶,参与多种生理及病理过程调控,其表达水平与神经退行性疾病、肿瘤发生等研究密切相关。试剂盒检测范围覆盖62.5 pg/mL至4000 pg/mL,实验操作全程约需4小时,包含预包被特异性抗体的酶标板、标准品、检测抗体及显色液等核心组分,具备高灵敏度和特异性。样本需预先离心处理,避免反复冻融,建议实验前通过预稀释确定样本适用浓度。该试剂盒可广泛应用于基础科研领域,如凋亡信号通路机制研究、药物开发中的靶点验证或疾病模型中Caspase-9活性变化的动态监测,为体外实验提供稳定可靠的定量分析工具。
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缩写:Casp-9
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Uniprot No.:
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种属:Rattus norvegicus (Rat)
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样本类型:serum, plasma, tissue homogenates
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检测范围:62.5 pg/mL-4000 pg/mL
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灵敏度:15.6 pg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Cell Biology
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat Casp-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 96 Range % 90-99 1:2 Average % 95 Range % 91-102 1:4 Average % 98 Range % 94-103 1:8 Average % 102 Range % 96-106 -
回收率:
The recovery of rat Casp-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 89-98 EDTA plasma (n=4) 100 96-104 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 4000 1.846 1.946 1.896 1.752 2000 1.442 1.542 1.492 1.348 1000 0.986 1.016 1.001 0.857 500 0.546 0.556 0.551 0.407 250 0.320 0.330 0.325 0.181 125 0.266 0.278 0.272 0.128 62.5 0.204 0.207 0.206 0.062 0 0.143 0.144 0.144 -
数据处理:
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货期:3-5 working days
引用文献
- Protective Effect of Whey Protein Supplement Against Rotenone Induced Motor Dysfunction in a Rat Model of Parkinson Disease P Zarei, B Amirpour-Najafabadi,Advanced Biomedical Research,2024
- Echis ocellatus venom-induced sperm functional deficits, pro-apoptotic and inflammatory activities in male reproductive organs in rats: antagonistic role of kaempferol BS Ajisebiola, AA Toromade, JO Oladele,BMC Pharmacology and Toxicology,2024
- Rhoifolin protects cisplatin mediated pulmonary toxicity via attenuation of oxidative stress, inflammatory response, apoptosis and histopathological damages A Akbar,JOURNAL OF KING SAUD UNIVERSITY SCIENCE,2024
- Cardio-nephrotoxicity mediated by Echis ocellatus venom and its amelioration through kaempferol’s suppressive effect on oxidative stress, inflammation, and apoptosis expression BS Ajisebiola,Clinical Phytoscience,2024
- Diosmin protects the testicles from doxorubicin-induced damage by increasing steroidogenesis and suppressing oxido-inflammation and apoptotic mediators MO Oyovwi,International journal of biochemistry and molecular biology,2024
- Evaluation of Possible Ameliorative Role of Robinetin to Counteract Polystyrene Microplastics Instigated Renal Toxicity in Rats MU Ijaz,/,2024
- Ameliorative Effect of Thymoquinone and Thymoquinone Nanoparticles against Diazinon-Induced Hepatic Injury in Rats: A Possible Protection Mechanism WM Nassar,Toxics,2023
- Exposure to bromoxynil octanoate herbicide induces oxidative stress, inflammation, and apoptosis in testicular tissue via modulating NF-кB pathway MMF El-Nagar,Food and chemical toxicology,2023
- Parthenolide repressed endometriosis induced surgically in rats: Role of PTEN/PI3Kinase/AKT/GSK-3β/β-catenin signaling in inhibition of epithelial mesenchymal transition SL Kabil,Life sciences,2023
- Ameliorative potential of sinensetin against paraquat induced renal damage by regulating oxidative inflammatory apoptotic and histopathological profile in male albino rats J Zhao,/,2024
- Simvastatin ameliorates testosterone-induced prostatic hyperplasia in rats via modulating IGF-1/PI3K/AKT/FOXO signaling NH El-Shafei,European journal of pharmacology,2023
- Therapeutic Efficacy of Chrysoeriol on Doxorubicin-induced Liver Damage by Improving Biochemical and Histological Profile in Rats MU Ijaz,SciEP,2023
- Evaluation of the Possible Protective Role of Nobiletin against Arsenic-Induced Liver Damage in Male Albino Rats MU Ijaz,Toxics,2023
- Diosmetin alleviates nonylphenol-induced liver damage by improving biochemical, inflammatory, apoptotic and histological profile in rats R Azmat,Journal of King Saud University - Science,2022
- Fisetin Attenuates Arsenic-Induced Hepatic Damage by Improving Biochemical, Inflammatory, Apoptotic, and Histological Profile: In Vivo and In Silico Approach M Umar,Evidence-Based Complementary and Alternative Medicine,2022
- Methanol Extract of Chasmanthera dependens Stem Mitigates against Mechanisms Involved in Piroxicam-induced Liver Damage in Rat TS Abiola,Journal of Complementary and Alternative Medical Research,2020
- Formononetin Upregulates Nrf2/HO-1 Signaling and Prevents Oxidative Stress, Inflammation, and Kidney Injury in Methotrexate-Induced Rats Aladaileh S H, et al,Antioxidants,2019
- Bacoside a Attenuates Nephrotoxicity and Acute Kidney Injury in Male Albino Rats Induced by Cisplatin Gong Xiao, et al,International Journal of Pharmacology,2019
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