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Rat Caspase-9,Casp-9 ELISA Kit

  • 中文名称:
    大鼠胱天蛋白酶9(Casp-9)酶联免疫试剂盒
  • 货号:
    CSB-E08863r
  • 规格:
    96T/48T
  • 价格:
    ¥3900/¥2500
  • 其他:

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  • 产品描述:
    CUSABIO特异性检测大鼠胱天蛋白酶9(Caspase-9)的定量ELISA试剂盒,采用双抗体夹心法原理,适用于血清、血浆及组织匀浆样本的检测。Caspase-9是半胱氨酸蛋白酶家族成员,在细胞凋亡的线粒体途径中作为关键启动酶,参与多种生理及病理过程调控,其表达水平与神经退行性疾病、肿瘤发生等研究密切相关。试剂盒检测范围覆盖62.5 pg/mL至4000 pg/mL,实验操作全程约需4小时,包含预包被特异性抗体的酶标板、标准品、检测抗体及显色液等核心组分,具备高灵敏度和特异性。样本需预先离心处理,避免反复冻融,建议实验前通过预稀释确定样本适用浓度。该试剂盒可广泛应用于基础科研领域,如凋亡信号通路机制研究、药物开发中的靶点验证或疾病模型中Caspase-9活性变化的动态监测,为体外实验提供稳定可靠的定量分析工具。
  • 缩写:
    Casp-9
  • Uniprot No.:
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    62.5 pg/mL-4000 pg/mL
  • 灵敏度:
    15.6 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cell Biology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Casp-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %96
    Range %90-99
    1:2Average %95
    Range %91-102
    1:4Average %98
    Range %94-103
    1:8Average %102
    Range %96-106
  • 回收率:
    The recovery of rat Casp-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)10096-104
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40001.846 1.946 1.896 1.752
    20001.442 1.542 1.492 1.348
    10000.986 1.016 1.001 0.857
    5000.546 0.556 0.551 0.407
    2500.320 0.330 0.325 0.181
    1250.266 0.278 0.272 0.128
    62.50.204 0.207 0.206 0.062
    00.143 0.144 0.144  
  • 数据处理:
  • 货期:
    3-5 working days

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