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  4. Rat Caspase-1(CASP1) ELISA kit

Rat Caspase-1(CASP1) ELISA kit

文献引用 (11) 说明书下载
  • 中文名称：
    大鼠半胱氨酸天冬氨酸蛋白酶1(CASP1)酶联免疫试剂盒
  • 货号：
    CSB-EL004543RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO大鼠半胱氨酸天冬氨酸蛋白酶1（CASP1）定量检测试剂盒，采用双抗体夹心法原理开发，适用于科研领域对血清、血浆、细胞裂解液及细胞培养上清样本中CASP1蛋白含量的精准检测。CASP1作为半胱氨酸蛋白酶家族成员，在细胞焦亡和炎症小体激活中发挥关键作用，其表达水平变化与炎症反应、神经退行性疾病等病理过程密切相关。该试剂盒灵敏度覆盖6.25至400 pg/mL的检测范围，可有效捕捉样本中微量目标蛋白，实验操作全程约需4.5小时，提供预包被96孔板、标准品及配套试剂等完整组件。研究人员可将其应用于炎症机制研究、药物干预效果评估或细胞模型中CASP1动态变化的监测，特别在神经免疫、感染性疾病模型构建及免疫调节剂开发等基础研究中具有重要价值，所有实验数据均需结合其他分子生物学方法进行综合分析。产品经过严格的质检流程，确保批间稳定性与检测准确性，为科研工作者提供可靠的技术支持。
  • 别名：
    Casp1 ELISA kit; Il1bc ELISA kit; Il1bceCaspase-1 ELISA kit; CASP-1 ELISA kit; EC 3.4.22.36 ELISA kit; Interleukin-1 beta convertase ELISA kit; IL-1BC ELISA kit; Interleukin-1 beta-converting enzyme ELISA kit; ICE ELISA kit; IL-1 beta-converting enzyme ELISA kit; p45) [Cleaved into: Caspase-1 subunit p20; Caspase-1 subunit p10] ELISA kit
  • 缩写：
    CASP1
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell lysates, cell culture supernates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CASP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 91    
    Range % 88-93    
    1:2 Average % 95    
    Range % 90-102    
    1:4 Average % 97    
    Range % 94-101    
    1:8 Average % 94    
    Range % 91-98    
  • 回收率：
    The recovery of rat CASP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 90 86-94    
    EDTA plasma (n=4) 99 93-105    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    400 2.189 2.321 2.255 2.047    
    200 1.729 1.754 1.742 1.534    
    100 1.151 1.165 1.158 0.950    
    50 0.755 0.748 0.752 0.544    
    25 0.456 0.468 0.462 0.254    
    12.5 0.317 0.325 0.321 0.113    
    6.25 0.277 0.290 0.284 0.076    
    0 0.201 0.215 0.208 　    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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Casp1 Proteins

靶点详情

  • 功能：
    Thiol protease involved in a variety of inflammatory processes by proteolytically cleaving other proteins, such as the precursors of the inflammatory cytokines interleukin-1 beta (IL1B) and interleukin 18 (IL18) as well as the pyroptosis inducer Gasdermin-D (GSDMD), into active mature peptides. Plays a key role in cell immunity as an inflammatory response initiator: once activated through formation of an inflammasome complex, it initiates a proinflammatory response through the cleavage of the two inflammatory cytokines IL1B and IL18, releasing the mature cytokines which are involved in a variety of inflammatory processes. Cleaves a tetrapeptide after an Asp residue at position P1. Also initiates pyroptosis, a programmed lytic cell death pathway, through cleavage of GSDMD. In contrast to cleavage of interleukins IL1B and IL1B, recognition and cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive. In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly.
  • 亚细胞定位：
    Cytoplasm. Cell membrane.
  • 蛋白家族：
    Peptidase C14A family
  • 数据库链接：

    STRING: 10116.ENSRNOP00000009993

    UniGene: Rn.37508

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