Rat Carcinoembryonic Antign,CEA ELISA Kit

产品详情

产品描述：

CUSABIO专为大鼠样本设计的癌胚抗原（CEA）定量检测试剂盒，采用双抗体夹心法ELISA技术，适用于血清、血浆、组织匀浆及细胞裂解液等多种样本类型。CEA是一种重要的糖蛋白类肿瘤标志物，在多种肿瘤发生发展中呈现高表达，广泛用于癌症机制研究及实验动物模型中的肿瘤进展监测。该试剂盒检测灵敏度为0.312 ng/mL，线性范围覆盖0.312-20 ng/mL，可精准量化样本中CEA的浓度水平。其高特异性抗体组合确保了对大鼠CEA的靶向识别，适用于体外研究肿瘤微环境、评估抗癌药物干预效果或筛选潜在生物标志物等科研场景。试剂盒提供标准化的预包被板条及配套试剂，操作流程简便，兼容常规实验室设备，支持批量样本的高通量分析，为肿瘤生物学研究、药理毒理实验及基础医学探索提供可靠工具。实验全程需严格参照说明书进行样本前处理及数据计算，结果仅限科研用途。
别名：

N/A
缩写：

CEA
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CEA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of rat CEA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	86	80-89

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.